Ham’s F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells.
Ecose™ Ham’s F-12 Nutrient Mix, Glutamine Supplement
Price range: $46.84 through $433.00
Purchase this item and get Product Description
Ham’s F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Ecose™ F-12 modifications for a range of cell culture applications.
This Ham’s F-12 is modified as follows:
With: Glutamine, Phenol Red
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations. Ecose™ F-12 with Glutamine supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
Ecose™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Content And Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Type
Primary Rat Astrocytes
Concentration
1 X
Product Type
Ham’s F-12 Nutrient Mixture
Sterility
Sterile-filtered
Cell Line
CHO, COS-7, and rat prostate epithelial cells
Classification
Animal Origin-free
Form
Liquid
With Additives
Glutamine, Phenol Red, Sodium Pyruvate
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
500 mL
| Product Quantity | 500 mL, 10 x 500 mL |
|---|
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