Ecose™ Ham's F-12 Nutrient Mix

Ecose™ Ham’s F-12 Nutrient Mix

Price range: $37.00 through $320.00

Ham’s F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.

Purchase this item and get 3-32 Points
Purchase this item and get 3-32 Points
Product Description

Ham’s F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. 

This Ham’s F-12 is modified as follows:
With: L-glutamine, Phenol Red

The complete formulation is available.

Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Content And Storage

Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Type

Primary Rat Astrocytes

Concentration

1 X

Product Type

Ham’s F-12 Nutrient Mixture

Sterility

Sterile-filtered

Cell Line

CHO, COS-7, and rat prostate epithelial cells

Classification

Animal Origin-free

Form

Liquid

With Additives

Glutamine, Phenol Red, Sodium Pyruvate

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Quantity

500 mL

Product Quantity

500 mL, 10 x 500 mL, 1000 mL, 6 x 1000 mL
SKU: CBM8949 Category:
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