Ecose™ Ham's F-10 Nutrient Mix, HEPES

Ecose™ Ham’s F-10 Nutrient Mix, HEPES

$58.25

Ham’s F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells.

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Product Description

Ham’s F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

This DMEM/F-12 is modified as follows:
With: Phenol Red, HEPES, L-glutamine

The complete formulation is available.

Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham’s F-12 Nutrient Mixture (F-12).

Using Ham’s F-10 Nutrient Mixture
Ecose™ F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Content And Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Type

Primary Rat Astrocytes

Concentration

1 X

Product Type

Ham’s F-10 Nutrient Mixture

Sterility

Sterile-filtered

Cell Line

CHO, COS-7, and rat prostate epithelial cells

Classification

Animal Origin-free

Form

Liquid

With Additives

High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Quantity

500 mL

Product Quantity

500 mL
SKU: CBM6445 Category:
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