DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Ecose™ DMEM, high glucose, no glutamine
Price range: $33.34 through $287.00
Purchase this item and get Product Description
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications.
This DMEM is modified as follows:
With: High Glucose, Phenol Red
Without: L-glutamine, Sodium Pyruvate, HEPES
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Content And Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Type
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Concentration
1 X
Product Type
DMEM (Dulbecco’s Modified Eagle Medium)
Sterility
Sterile-filtered
Cell Line
HeLa, 293, Cos-7, and PC-12
Classification
Animal Origin-free
Form
Liquid
Serum Level
Placeholder
With Additives
High Glucose, Phenol Red
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
500 mL
| Product Quantity | 500 mL, 10 x 500 mL, 1000 mL, 6 x 1000 mL |
|---|
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