DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Ecose™ DMEM/F-12, powder, HEPES
$114.00
Purchase this item and get Product Description
DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications.
This DMEM/F-12 is modified as follows:
With: L-glutamine, HEPES, Phenol Red
Without: Sodium Bicarbonate
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins with F-12’s wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Ecose™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.
Content And Storage
Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Product Type
DMEM (Dulbecco’s Modified Eagle Medium)/F-12
Sterility
Sterile-filtered
Cell Line
MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Classification
Animal Origin-free
Form
Powder
Serum Level
Standard Serum Supplementation
With Additives
High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
10 x 1 L
| Product Quantity | 10 x 1 L |
|---|
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