Dulbecco’s Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Dulbecco’s Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Ecose™ DMEM/F-12 modifications for a range of cell culture applications.
This DMEM/F-12 is modified as follows:
With: Phenol Red
Without: L-glutamine, HEPES
Ecose™ DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids and vitamins with F-12’s wide variety of components.
DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Mammalian Cells
1 X
Dulbecco’s Modified Eagle Medium (DMEM)
Sterile-filtered
Liquid
Standard Serum Supplementation
High Glucose, Phenol Red, Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
500 mL
Product Quantity | 500 mL |
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