Ecose™ DMEM/F-12, Glutamine supplement

Ecose™ DMEM/F-12, Glutamine supplement

$62.75

DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Purchase this item and get 6 Points
Purchase this item and get 6 Points
Product Description

DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. 

This DMEM / F-12 is modified as follows:
With: Glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins with F-12’s wide variety of components. DMEM/F-12 with Glutamine supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Content And Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Concentration

1 X

Product Type

DMEM (Dulbecco’s Modified Eagle Medium)/F-12

Sterility

Sterile-filtered

Cell Line

MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts

Classification

Animal Origin-free

Form

Liquid

Serum Level

Standard Serum Supplementation

With Additives

High Glucose, Glutamine, Phenol Red, Sodium Pyruvate

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Quantity

500 mL

Product Quantity

500 mL
SKU: CBM2531 Category:
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