The ClonePro™ PCR Cloning Kits efficiently enables ready-to-use ligation reactions. It requires only a blazingly-quick 40 minutes after the PCR to plated cells, hence it is less time-consuming. It consists of SOC medium and transformed competent cells that can be immediately plated as well. It is highly specific due to UA hybridization that enables efficient cloning.
ClonePro™ PCR Cloning Kits
$562.00
Purchase this item and get Product Description
The ClonePro™ PCR Cloning Kits represent a breakthrough in molecular cloning, offering an efficient and user-friendly platform for ready-to-use ligation reactions. Significantly reducing the time required for the entire process, a mere 40 minute elapse from PCR to plated cells, making it an exceptionally time-conscious choice. This kit includes SOC medium and transformed competent cells, which can be promptly plated without the customary recovery incubation, further enhancing its efficiency.
The high specificity of the ClonePro™ PCR Cloning Kit is attributed to UA hybridization, a mechanism that ensures a remarkably efficient cloning process. Leveraging cutting-edge ligation technology, the kit excels in the rapid and highly efficient cloning of PCR products, especially those generated by Taq and other nonproofreading DNA polymerases. Rigorous performance testing against other suppliers’ kits establishes its superiority, demonstrating successful results and accelerated cloning into the TurboClone™ Cloning Vector when compared to traditional TA-based vectors. The TurboClone Cloning Vector, a key component of this kit, employs UA hybridization to achieve highly efficient cloning of PCR products and contains ampicillin and kanamycin sites. Its linear form with U overhangs specifically hybridizes with A overhangs found in PCR products from Taq and similar DNA polymerases. This vector is equipped with unique features such as multiple restriction enzyme sites, universal sequencing primer sites, and dual antibiotic selection, facilitating faster and more specific cloning than TA-based vectors.
The Ligation Master Mix and SwiftCells™ Competent Cells seamlessly integrate into the process, streamlining efficiency without the need for recovery incubation. In the simplified procedure, the PCR product is mixed with TurboClone™ Cloning Vector and Ligation Master Mix, followed by incubation, and the ligation mixture is directly added to SwiftCells™ Competent Cells for immediate transformation, eliminating the need for recovery incubation. The entire ClonePro™ PCR Cloning Kit procedure is impressively swift, with a 30-minute ligation and a 10-minute transformation and plating using SwiftCells Competent Cells, resulting in a comprehensive process from PCR product to plated cells within just 40 minutes. These findings collectively underscore the kit’s outstanding features, superior performance, underlying principles, and streamlined procedural efficiency.
Kit Contents
For 40 reactions
2X Ligation Master Mix (1 X 0.2mL)
TurboClone™ Cloning Vector (2.0 µg)
Distilled water (1.7 mL)
(Media and competent cells are supplied with ClonePro Ultra Cloning Kit)
Technical Parameters
Master Mix format for PCR
30 minute ligation procedure
10^8 CFU for SwfitCells
Kanamycin and Ampcillin sites the TurboClone™ vector
Several Unique restriction sites
Applications
The kit is applicable to all your bacterial cloning needs.
Product FAQs
How does the UA hybridization principle contribute to the high efficiency of cloning in the TurboClone™ Cloning Vector?
The UA hybridization principle enhances cloning efficiency in the TurboClone™ Cloning Vector by providing a specific U-A pairing between vector U overhangs and A overhangs in PCR products, minimizing non-specific interactions.
What makes the ligation technology in this kit cutting-edge, and how does it enhance the speed and efficiency of the cloning process?
The ligation technology in the ClonePro™ PCR Kit is cutting-edge due to its rapid and efficient performance, enabling a 30-minute ligation time and contributing to overall procedural speed and efficiency.
How does the ClonePro™ Cloning Kit address the issue of false-positive colonies, particularly in comparison to TA-based cloning vectors?
The ClonePro™ PCR Cloning Kit minimizes false-positive colonies through its UA hybridization principle, reducing the likelihood of non-specific interactions and self-annealing.
Can the Ligation Master Mix be purchased separately, and is it compatible with other cloning systems?
The Ligation Master Mix is not sold separately, and its compatibility with other cloning systems may vary.
| Reactions | 40 |
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