Plasmid Buffers are essential for plasmid DNA purification, ensuring high yield and purity for downstream applications such as cloning, sequencing, and transfection.
Buffers for Plasmid Isolation
Price range: $102.00 through $174.00
Purchase this item and get Product Description
Plasmid Buffers are formulated to provide optimal conditions for the purification of plasmid DNA from bacterial cultures. These buffers are essential in ensuring high yield and purity of plasmid DNA, which is crucial for various molecular biology applications, including cloning, sequencing, and transfection. The set typically includes resuspension buffer, lysis buffer, and neutralization buffer, each playing a vital role in the plasmid extraction process. The efficient purification process enabled by these buffers helps in obtaining plasmid DNA of high integrity, free from contaminants that could interfere with downstream applications. Plasmid Buffers are compatible with various plasmid purification kits and protocols, making them versatile and user-friendly. The high-quality reagents ensure reproducibility and reliability, essential for research and clinical laboratories aiming to achieve consistent results in their plasmid DNA workflows.
Kit Contents
● Resuspension Buffer (500 mL)
● Lysis Buffer (500 mL)
● Neutralization Buffer 1 & 2 (500 mL each)
● Wash Buffer (1000 mL)
● Equilibration Buffer (1000 mL)
● Elution Buffer (1000 mL)
Technical Parameters
● Contains RNase A for RNA degradation
● Alkaline lysis buffer for cell disruption
● Neutralization buffer for plasmid renaturation
● Ensures high yield and purity of plasmid DNA
Applications
● Plasmid DNA purification
● Cloning
● Sequencing
● Transfection
● Downstream molecular biology applications
Product FAQs
What are Plasmid Buffers used for?
They are used for purifying plasmid DNA from bacterial cultures.
Why is RNase A included in Resuspension Buffer?
RNase A degrades RNA, ensuring cleaner plasmid preparations.
What downstream applications can the purified plasmid DNA be used for?
It can be used for cloning, sequencing, transfection, and other molecular biology applications.
| Quantity | Elution Buffer: 1000mL, Equilibration Buffer: 1000mL, Lysis Buffer: 500mL, Neutralization Buffer 1 & 2: 500mL each, Resuspension Buffer: 500mL, Wash Buffer: 1000mL |
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