Obtain high yields of transfection-grade plasmid DNA conveniently in a 96-well format with Avam® Vac Plasmid Plate Kits.
Avam® Vac Plasmid Plate Kits
$3,445.00
Purchase this item and get Product Description
Avam® Vac Plasmid Plate Kits offer a state-of-the-art solution for plasmid DNA purification in a user-friendly 96-well plate format. These kits utilize a proprietary binding chemistry to deliver transfection-grade plasmid DNA with endotoxin levels reduced to <1 EU/µg DN, ensuring superior performance in downstream applications. The procedure is streamlined and efficient, employing a straightforward bind-wash-elute process that yields up to 50 µg of high-quality plasmid DNA per well from E. coli cultures. Compatible with vacuum, centrifugation, or automated processing options, these kits provide flexibility to accommodate diverse laboratory setups. Whether you’re performing transfection experiments, cloning, or enzymatic modifications, rely on Avam® Vac Plasmid Plate Kits for consistent and reproducible results.
Kit Contents
● Avam® Vaccum Filter 96 Well Plates
● Buffers
● Reagents
● Square Well Blocks
● Elution Microtubes
Technical Parameters
● Plasmid type: High-copy, low-copy, cosmid DNA
● Processing: Manual (gravity flow)
● Sample per run: 1 sample per run
● Yield: <20 µg (Mini Kit), up to 100 µg (Midi Kit), <500 µg (Maxi Kit),
<2.5 mg (Mega Kit), <10 mg (Giga Kit)
● Time per run: 80 min (Mini Kit), 150 min (Midi Kit), 160 min (Maxi Kit),
220 min (Mega Kit), 320 min (Giga Kit)
Applications
● Transfection
● Cloning
● PCR
● In vitro transcription
Product FAQs
What is the endotoxin level in the purified plasmid DNA?
The endotoxin level is reduced to <1 EU/µg DNA.
What is the maximum yield of plasmid DNA per well?
Up to 50 µg of plasmid DNA can be obtained per well from a 5 mL E. coli culture.
Are these kits compatible with vacuum filtration systems?
The purification procedure typically takes around 1.5 to 2 hours, depending on the sample type and processing conditions.
Can the purified DNA be used for long-read sequencing applications?
Yes, the kit components are compatible with all vacuum filtration systems.
What types of plasmid DNA can be purified using these kits?
These kits are suitable for purifying high-copy, low-copy, and cosmid DNA.
| Quantity | 4 U |
|---|
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