Experience rapid purification of up to 24 samples of highly pure plasmid DNA with the Avam® Vac Plasmid Kits, featuring fast vacuum-based parallel processing and low elution volumes for concentrated DNA.
Avam® Vac Plasmid Kits
Price range: $82.00 through $3,528.00
Purchase this item and get Product Description
The Avam® Vac Plasmid Kits offer a cutting-edge solution for ultrafast, large-scale purification of highly pure plasmid DNA. With the ability to process up to 24 samples simultaneously, these kits utilize vacuum-based technology for rapid lysate clearing, significantly reducing hands-on time. The purified DNA, obtained in low elution volumes, is of exceptional quality, with endotoxin levels reduced to <1 EU/μg due to the dedicated wash buffer included in the kit. The resulting plasmid DNA is ideal for a wide range of applications, including transfection into sensitive cell lines. For optimal results, these kits are recommended for use with the QIAvac 24 Plus vacuum manifold.
Kit Contents
● Avam® Vaccum 96 Plates
● Buffers
● Reagents
● Square-Well Blocks
● Elution Microtubes
Technical Parameters
● Culture volume/starting material: 2500 ml (Giga Kit), 500 ml (Mega Kit), 50–200 ml (Maxi Kit), 25–50 ml (Midi Kit)
● Final DNA concentration: ≥1 μg/μl
● Samples per run: 1–12 samples per run (Giga and Mega Kits), 1–24 samples per run (Maxi and Midi Kits)
● Time per run: 50 min (Giga Kit), 40 min (Mega Kit), 20 min (Maxi and Midi Kits)
● Yield: Upto 10mg (GigaKit), up to 2.5mg (MegaKit), up to 1mg (Maxi Kit), up to 250 μg (Midi Kit)
Applications
● Transfection into most cell lines
● Restriction digestion
● Cloning
● In vitro transcription and translation
● Sequencing
Product FAQs
What is the endotoxin level in the purified plasmid DNA?
The endotoxin level is reduced to <1 EU/μg DNA.
What is the maximum yield of plasmid DNA per well?
Up to 10 mg of plasmid DNA can be obtained per well from a 5 ml E. coli culture.
Are these kits compatible with vacuum filtration systems?
Yes, the kit is compatible with all types of vacuum filtration systems.
What types of plasmid DNA can be purified using these kits?
These kits are suitable for purifying plasmids and cosmids.
| Kit / Components | Midi Plasmid, Maxi Plasmid, Mega Plasmid, Giga Plasmid, Holder for Columns, Vacuum Valve Setup |
|---|---|
| Quantity | 5 units, 25 units, 100 units, 24 valves, N/A |
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