Effectively remove uracil from DNA with the Avam® Uracil DNA Glycosylase, ensuring accurate downstream molecular biology applications.
Avam® Uracil DNA Glycosylase
$474.00
Purchase this item and get Product Description
Avam® Uracil DNA Glycosylase performs as a vital enzyme for molecular biology applications, catalyzing the hydrolysis of the N-glycosylic bond between uracil and the sugar, creating an abasic site in uracil-containing DNA. This enzyme is highly specific and exhibits no measurable activity on short oligonucleotides (<6 bases) or RNA substrates. Supplied in a convenient formulation, the enzyme is stable and active in most molecular biology reaction buffers, eliminating the need for buffer exchange. The 10X UDG Reaction Buffer ensures optimal enzymatic activity, maintaining the enzyme’s efficacy across various experimental conditions. Trusted for its high purity and specificity, the Avam® Uracil DNA Glycosylase is essential for applications requiring precise uracil removal from DNA molecules.
Kit Contents
● Avam® Uracil DNA Glycosylase (5.0 mL at 2000 U/mL)
● 10x UDG Reaction Buffer (3 X 1.5 mL)
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 25.6 Kilo Daltons
● Purity: >95%
● Specific activity: 77,000 U/mg
Applications
● Carryover prevention in PCR reactions
● Creating abasic sites in single- or double-stranded DNA
Product FAQs
What is the storage temperature for the Avam® Uracil DNA Glycosylase?
The Avam® Uracil DNA Glycosylase should be stored at temperatures between -25°C to -15°C for optimal stability and activity.
Can the Avam® Uracil DNA Glycosylase be used with other molecular biology reaction buffers?
Yes, the enzyme is active in most molecular biology reaction buffers, eliminating the need for buffer exchange.
How is the specific activity of the Avam® Uracil DNA Glycosylase determined?
The specific activity is measured using a twofold serial dilution method, where dilutions of the enzyme are added to reactions containing a 3H-dUTP PCR product and 1x UDG Reaction Buffer. The reactions are then incubated and analyzed using a TCA-precipitation method.
Is the Avam® Uracil DNA Glycosylase suitable for preventing carryover in PCR reactions?
Yes, the enzyme can efficiently remove uracil from DNA, making it suitable for preventing carryover contamination in PCR reactions.
How can I inactivate the Avam® Uracil DNA Glycosylase if needed?
Heating at 95°C for 10 minutes only partially inactivates the enzyme. For full inactivation, a uracil glycosylase inhibitor should be added, or the reaction can be cleaned up using appropriate methods.
Is the Avam® Uracil DNA Glycosylase suitable for creating abasic sites in DNA?
Yes, the enzyme catalyzes the hydrolysis of the N-glycosylic bond between uracil and the sugar, creating abasic sites in uracil-containing DNA, making it suitable for such applications.
| Quantity | 10000U |
|---|
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