$474.00
The Uracil Cleavage System combines Uracil DNA Glycosylase (UDG) and Endonuclease VIII in a two-enzyme system, ideal for the precise excision of uracil bases from DNA substrates, creating single-nucleotide gaps essential for various molecular biology applications.
The Avam® Uracil Cleavage System facilitates the precise excision of uracil bases from DNA substrates, creating single-nucleotide gaps essential for various molecular biology applications. This two-enzyme system includes Uracil DNA Glycosylase (UDG) and Endonuclease VIII, each playing a distinct role in the process. UDG initiates the excision process by catalyzing the removal of uracil bases, resulting in the formation of an abasic site while preserving the integrity of the DNA backbone. Subsequently, Endonuclease VIII acts on the abasic site, cleaving the phosphodiester backbone both 3′ and 5′ to the uracil residue, thereby liberating the deoxyribose sugar. Together, these enzymes enable precise manipulation of DNA substrates, allowing researchers to create targeted modifications necessary for a wide range of molecular biology experiments, including cloning, DNA repair studies, and site-directed mutagenesis. The Avam® Uracil Cleavage System offers exceptional efficiency, specificity, and versatility, making it an invaluable tool for researchers working in the field of molecular biology.
● Uracil DNA Glycosylase (UDG) (0.375 mL)
● Endonuclease VIII (0.375 mL)
● Storage temperature: –25°C to –15°C
● Purity: >99%
● Single-stranded exonuclease: <5% released
● Double-stranded exonuclease: <1% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
● Cloning
● DNA manipulation in molecular biology experiments
The recommended storage temperature is between -25°C to-15°C.
The shelf life of the Avam® Uracil Cleavage System is typically several months when stored under recommended conditions.
Yes, the reaction volume can be adjusted while maintaining the ratios of components outlined in the usage instructions.
Yes, the system is compatible with various DNA substrates containing uracil bases.
The cleavage reaction can be stopped by adding a gel-loading stop solution or by proceeding with downstream applications after the specified incubation period.
| Quantity | 10X |
|---|
Purchase this item and get