The Avam® UDGase or DNA Uracil Glycosylase is a powerful tool for molecular biology applications, designed to catalyze the removal of uracil from uracil-containing DNA, ensuring the integrity of DNA substrates in various experimental procedures.
Avam® UDGase
Price range: $120.00 through $550.00
Purchase this item and get Product Description
The Avam® UDGase enzyme is a vital tool for molecular biology applications, specifically designed to catalyze the release of uracil from uracil-containing single-stranded or double-stranded DNA, leaving an apyrimidinic site in its wake. This modified recombinant enzyme, overexpressed in E. coli, offers heightened sensitivity, with a mutant variant rendered irreversibly inactive after a brief heating period, ensuring precise and controlled enzymatic activity.
Supplied with a comprehensive formulation including Tris-Cl, NaCI, EDTA, DTT, stabilizers, and glycerol, the Avam® UDGase exhibits robust activity over a broad pH range, with optimal performance observed at pH 8.0. Notably, this enzyme demonstrates exquisite specificity, displaying no activity on RNA or oligonucleotides, thereby ensuring the integrity of DNA substrates during enzymatic treatment.
Each unit of the enzyme is defined by its ability to catalyze the release of 60 pmol of uracil per minute from uracil-containing dsDNA, as measured by the release of [3H]-uracil in a standardized reaction setup. Rigorous quality control measures ensure the absence of unspecific DNA nucleases, guaranteeing the reliability and consistency of the enzyme across experiments.
Practically, the Avam® UDGase finds versatile applications in various molecular biology tasks, including the elimination of carry-over contamination PCR, probing for protein-DNA interactions, facilitating glycosylase-mediated single nucleotide polymorphism detection (GMPD), and aiding in the molecular cloning of PCR products. Its ease of use and in exceptional performance make it an indispensable asset for researchers seeking precise manipulation of DNA substrates in their experimental workflows.
Kit Contents
● Avam® UDGase enzyme: 500 μL at 1 U/μL
● 10x UDGase Reaction Buffer: 1 mL
Technical Parameters
● Storage temperature: -20°C
● Enzyme inactivation: Irreversible at 95°C for 10 minutes
● Unit definition: Release of 60 pmol of uracil per minute from uracil-containing dsDNA in a 50 μL reaction containing 0.2 μg DNA at 37°C
Applications
● Elimination of carry-over contamination in PCR
● Probe for protein-DNA interaction studies
● Glycosylase-mediated single nucleotide polymorphism detection (GMPD)
● Molecular cloning of PCR products
Product FAQs
What is the storage temperature for the Avam® UDGase?
The components should be stored at -20°C without a defrost cycle to maintain enzyme stability.
How can I inactivate the enzyme?
The enzyme can be irreversibly inactivated by incubating at 95°C for 10 minutes.
What is the unit definition of the enzyme?
One unit of the enzyme catalyzes the release of 60 pmol of uracil per minute from uracil-containing dsDNA in a standardized reaction setup.
What are the primary applications of this enzyme?
The enzyme finds applications in eliminating carry-over contamination in PCR, probing protein-DNA interactions, facilitating glycosylase-mediated single nucleotide polymorphism detection (GMPD), and aiding in the molecular cloning of PCR products.
| Quantity | 2500U, 500U |
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