The Avam® Taq DNA Ligase offers a robust and thermostable solution for sealing nicks in duplex DNA, making it indispensable for various molecular biology applications.
Avam® Taq DNA Ligase
$645.00
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Avam® Taq DNA Ligase, an essential enzyme in molecular biology, facilitates the formation of phosphodiester bonds within duplex DNA molecules, sealing nicks and enabling the creation of continuous DNA strands. This includes Avam® Taq DNA Ligase with a concentration of 40,000 U/mL, ensuring efficient and reliable performance in a wide range of experimental setups. Complemented by the 10X DNA Ligase Buffer, meticulously formulated to provide an optimal environment for enzymatic activity, this simplifies experimental procedures and enhances experimental outcomes.
Ideal for high-temperature ligation, ligase chain reaction (LCR), and ligase detection reaction (LDR), the Avam® Taq DNA Ligase enables researchers to perform seamless DNA manipulations with confidence. Whether conducting routine cloning experiments or exploring advanced molecular techniques, this empowers scientists with the tools needed to achieve their research goals efficiently and effectively.
Kit Contents
● Avam® Taq DNA Ligase (40,000 U/mL)
● 10X DNA Ligase Buffer
Technical Parameters
● Storage temperature: -25°C to -15°C
● Molecular weight: 76.9 kilo Daltons
● Purity: >99%
● Specific activity: 400,000 U/mg
● Single-stranded exonuclease: <5.0% released
● Double-stranded exonuclease: <1.0% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
Applications
● High-temperature ligation
● Ligase chain reaction
● Ligase detection reaction
Product FAQs
What is the function of Avam® Taq DNA Ligase?
Avam® Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor.
What are the features of Avam® Taq DNA Ligase?
Avam® Taq DNA Ligase is thermostable, seals nicks in DNA, and discriminates against mismatch ligation.
What is the storage temperature for Avam® Taq DNA Ligase?
Avam® Taq DNA Ligase should be stored at temperatures ranging from -25°C to -15°C to maintain its stability and activity.
How is the specific activity of Avam® Taq DNA Ligase determined?
The specific activity of Avam® Taq DNA Ligase is determined to be 400,000 U/mg.
Can Avam® Taq DNA Ligase be used for high-temperature ligation?
Yes, Avam® Taq DNA Ligase is suitable for high-temperature ligation reactions.
What is the composition of the 10X Avam® Taq DNA Ligase Buffer?
The 10X Avam® Taq DNA Ligase Buffer contains 200 mM Tris-HCI, 250 mM KCI, 100 mM MgCl2, 5 mM NAD+, and 0.1% Triton X-100, with a pH of 7.6 at 25°C.
What quality control measures are employed for Avam® Taq DNA Ligase?
Quality control measures include activity assays, protein concentration determination, SDS-PAGE analysis for purity, and tests for exonuclease activity, endonuclease activity, and E. coli contamination.
| Quantity | 40000U |
|---|
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