Seamlessly join cohesive-end termini and repair single-stranded nicks in duplex DNA with the Avam® T7 DNA Ligase, a high-quality enzyme essential for molecular biology applications.
Avam® T7 DNA Ligase
$474.00
Purchase this item and get Product Description
Unlock the potential of your molecular biology experiments with our Avam® T7 DNA Ligase, designed for seamless and efficient DNA ligation. This high-quality enzyme catalyzes the formation of phosphodiester bonds between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA, making it an indispensable tool for a wide range of molecular biology applications.
Avam® T7 DNA Ligase excels in joining cohesive-end termini and repairing single-stranded nicks in duplex DNA. While it exhibits low efficiency in ligating blunt ends, the addition of high concentrations of PEG 6000 can stimulate its activity, enabling measurable results. Notably, under standard reaction conditions, Avam® T7 DNA Ligase cannot efficiently perform blunt-end DNA ligation, making it particularly suitable for scenarios where cohesive ends need to be joined while blunt ends are present.
Supplied in a meticulously formulated buffer, our Avam® T7 DNA Ligase ensures optimal enzyme activity and stability. The inclusion of the 2x Rapid Ligation Buffer, containing essential components such as Tris-HCI, MgCl₂, DTT, ATP, and PEG 6000, further enhances the efficiency of ligation reactions, resulting in reliable and reproducible outcomes.
Each unit of Avam® T7 DNA Ligase is meticulously tested to ensure exceptional purity and performance. With its defined unit activity and stringent quality control measures, you can trust our Avam® T7 DNA Ligase to deliver consistent and reliable results, empowering your research endeavors.
Whether you’re involved in cloning ligation, adding linkers or adapters to double-stranded DNA, or sealing nicks in double-stranded DNA, our Avam® T7 DNA Ligase provides the precision and efficiency you need to advance your molecular biology projects with confidence.
Kit Contents
● Avam® T7 DNA Ligase (900,000 U/mL)
● 2X Fast Ligation Buffer
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 41.1 Daltons
● Purity: >99%
● Specific activity: 3,000,000 U/mg
● Single-stranded exonuclease: <1% released
● Double-stranded exonuclease: <1% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
Applications
● Cloning ligation
● Adding linkers or adapters to double-stranded DNA
● Nick-sealing in double-stranded DNA
Product FAQs
Can the Avam® T7 DNA Ligase efficiently join blunt ends?
No, Avam® T7 DNA Ligase exhibits low efficiency in ligating blunt ends. However, adding high concentrations of PEG 6000 can stimulate its activity.
What is the recommended DNA molar ratio for sticky-end ligation using Avam® T7 DNA Ligase?
The recommended DNA molar ratio for sticky-end ligation, such as cut vector and insert, is 1:3 (vector: insert).
Can the Avam® T7 DNA Ligase ligate single-stranded DNA fragments?
No, Avam® T7 DNA Ligase cannot ligate single-stranded DNA fragments.
What are the storage conditions for the Avam® T7 DNA Ligase?
The Avam® T7 DNA Ligase should be stored at temperatures between -25°C to-15°C for optimal stability and activity.
| Quantity | 900000U |
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