Avam® T4 RNA Ligase II

Product Description

Elevate your molecular biology experiments with our Avam® T4 RNA Ligase II. Engineered to catalyze phosphodiester bond formation between a 5′ phosphate and 3′ hydroxyl termini of RNA, this enables precise sealing of nicks on double-stranded RNA molecules. Avam® T4 RNA Ligase II, a pivotal enzyme in RNA manipulation, offers exceptional versatility and reliability across a spectrum of molecular biology applications.

Avam® T4 RNA Ligase II demonstrates remarkable efficiency in ligating nicked double-stranded RNA, serving as an invaluable tool for joining RNA fragments in various experimental contexts. Moreover, its ATP-dependent activity ensures robust performance, facilitating seamless integration into diverse RNA manipulation workflows.

In addition to its primary function in nick ligation, Avam® T4 RNA Ligase II accommodates pre-adenylated substrates, enhancing flexibility in experimental design. This feature broadens the utility, allowing researchers to explore innovative approaches in RNA manipulation and downstream applications.

Supplied with our meticulously formulated 10X Ligation Buffer, this offers not only superior performance but also unparalleled convenience. The buffer’s optimized composition ensures optimal enzyme activity, providing reproducible results with minimal optimization required.

Empower your research with the precision and efficiency of our Avam® T4 RNA Ligase II, delivering exceptional performance and reliability for your molecular biology endeavors.

Kit Contents

● 4500 U of Avam® T4 RNA Ligase II (30,000 U/mL)
● 10X Ligation Buffer (1 x 1.5 mL)

Technical Parameters

● Storage temperature: –25°C to –15°C
● Molecular weight: 37.6 kilo Daltons
● Purity: >99%
● Specific activity: >120,000 U/mg
● Single-stranded exonuclease: <5.0 % released
● Double-stranded exonuclease: <1.0 % released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
● RNase contamination: No detectable non-specific RNase

Applications

● Nick ligation in double-stranded RNA
● Ligation of the 3′ OH of RNA to the 5′ phosphate of DNA in a
double-stranded-format NGS RNA library construction (miRNA-seq or
directional mRNA-seq)

Product FAQs

What is the preferred substrate for Avam® T4 RNA Ligase II?

Avam® T4 RNA Ligase II exhibits optimal activity with nicked double-stranded RNA, and can further ligate other nicked nucleic acid hybrids.

Does Avam® T4 RNA Ligase II require ATP for activity?

Yes, Avam® T4 RNA Ligase II requires ATP for its ligase activity, unless the substrate is pre-adenylated on the 5′ end.

How can I stop the ligation reaction?

The ligation reaction can be stopped by adding EDTA to a final concentration of 12.5 mM or by incubating at 65°C for 20 minutes.