Elevate your molecular biology assays with the Avam® T4 Polynucleotide Kinase, designed to efficiently label DNA or RNA molecules for a range of applications.
Avam® T4 Polynucleotide Kinase
$470.00
Purchase this item and get Product Description
The Avam® T4 Polynucleotide Kinase offers a comprehensive solution for phosphorylating the 5′ ends of DNA or RNA molecules, essential for various molecular biology applications such as labeling probes, transcript mapping, sequencing, and more. The Avam® T4 Polynucleotide Kinase (PNK) included in the kit catalyzes the transfer and exchange of phosphate groups, enabling efficient labeling of both single-stranded and double-stranded nucleic acids. Additionally, the enzyme exhibits 3′-phosphatase activity, allowing for the removal of 3′ phosphoryl groups from DNA and RNA molecules. The kit provides high-quality T4 PNK enzymes and optimized buffers, ensuring reliable and reproducible results in your experiments.
Kit Contents
● Avam® T4 Polynucleotide Kinase (10,000 U/mL)
● 10X Polynucleotide Kinase Buffer (2 x 1.5 mL)
Technical Parameters
● Purity: >99%
● Specific activity: 133,333 U/mg
● Single-stranded exonuclease: <5.0% released
● Double-stranded exonuclease: <1.0% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
Applications
● Labeling the 5′ ends of DNA or RNA for hybridization probes
● Transcript mapping with nucleases
● Sequencing applications
● Phosphorylating oligodeoxynucleotide linkers prior to ligation
● End-labeling oligodeoxynucleotide primers for sequencing reactions
● Dephosphorylating the 3′-ends of RNA
● Exchange reactions to label the 5′ ends of DNA or RNA molecules
Product FAQs
What is the role of Avam® T4 Polynucleotide Kinase in molecular biology assays?
Avam® T4 Polynucleotide Kinase catalyzes the transfer and exchange of phosphate groups, allowing for efficient labeling of DNA or RNA molecules, essential for various molecular biology applications.
Can this kit be used for end-labeling oligodeoxynucleotide primers?
Yes, the Avam® T4 Polynucleotide Kinase Labeling Kit can be used for end-labeling oligodeoxynucleotide primers for sequencing reactions.
Is the Avam® T4 Polynucleotide Kinase enzyme compatible with sequencing applications?
Yes, the Avam® T4 Polynucleotide Kinase enzyme is suitable for sequencing applications, allowing for efficient labeling of DNA or RNA molecules prior to sequencing.
| Quantity | 10000U |
|---|
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