Experience efficient DNA synthesis and modification with the Avam® T4 DNA Polymerase, featuring a highly versatile enzyme for molecular biology applications.
Avam® T4 DNA Polymerase
$474.00
Purchase this item and get Product Description
Elevate your molecular biology experiments with the Avam® T4 DNA Polymerase, a comprehensive solution designed to streamline DNA synthesis and modification processes. At the heart lies Avam® T4 DNA Polymerase, a robust enzyme derived from a recombinant E. coli strain. This enzyme exhibits remarkable polymerase activity, facilitating the extension of DNA primers in the 5′-3′ direction, while also boasting potent 3′-5′ exonuclease activity for meticulous proofreading. Whether you’re generating blunt ends, performing single-strand deletion subcloning, conducting site-directed mutagenesis, or labeling probes through replacement synthesis, this equips you with the tools for precise and reliable DNA manipulation. Additionally, it includes a specially formulated 10X Vivid Blue™, ensuring optimal conditions for Avam® T4 DNA Polymerase activity. Unlock new possibilities in your molecular biology research with our Avam® T4 DNA Polymerase.
Kit Contents
● Avam® T4 DNA Polymerase
● 10× VividBlue™
Technical Parameters
● Storage temperature: -25°C to -15°C
● Specific activity: 5555 U/mg
● Single-stranded exonuclease: Functional
● Double-stranded exonuclease: Functional
● Double-stranded endonuclease: 30 U; no conversion
● E. coli DNA contamination: <10 copies
Applications
● Generating blunt ends for 5′ or 3′ overhangs
● Single-strand deletion subcloning
● Second strand synthesis in site-directed mutagenesis
● Probe labeling using replacement synthesis
Product FAQs
What are the storage conditions for Avam® T4 DNA Polymerase?
Avam® T4 DNA Polymerase should be stored at -25°C to -15°C for optimal stability and activity.
What is the composition of the 10X VividBlue™ supplied?
The 10× VividBlue™ contains 500mM NaCl, 100mM Tris-HCl, 100mM MgCl2, 10mM DTT, pH 7.9 at 25°C.
Does Avam® T4 DNA Polymerase possess 5'-3' exonuclease activity?
No, Avam® T4 DNA Polymerase lacks 5′-3′ exonuclease activity, making it suitable for specific DNA manipulation applications.
What are the recommended applications for Avam® T4 DNA Polymerase?
Avam® T4 DNA Polymerase is commonly used for generating blunt ends, single-strand deletion subcloning, second strand synthesis in site-directed mutagenesis, and probe labeling using replacement synthesis.
How is the quality of Avam® T4 DNA Polymerase assessed?
The quality of Avam® T4 DNA Polymerase is evaluated through assays such as the twofold serial dilution method, gel electrophoresis for exonuclease activity, and detection of E.coli contamination using qPCR.
| Quantity | 2000U |
|---|
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