Simplify your molecular cloning processes with the comprehensive Avam® T4 DNA Ligase Molecular Biology Grade Kit, featuring high-quality components for seamless DNA ligation.
Avam® T4 DNA Ligase MBG
Price range: $54.60 through $179.00
Purchase this item and get Product Description
The Avam® T4 DNA Ligase Molecular Biology Grade is an essential tool for molecular biologists, enabling precise joining of DNA fragments with unparalleled efficiency. Derived from Escherichia coli, this ATP-dependent enzyme catalyzes the formation of phosphodiester bonds between 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA. Its versatility extends to joining both blunt-ended and cohesive-ended DNA fragments, along with repairing single-stranded nicks in various nucleic acid substrates, including duplex DNA, RNA, or DNA/RNA hybrids. Each component in the kit is meticulously formulated to ensure optimal performance and reliability in a wide range of molecular biology applications.
Kit Contents
● T4 DNA Ligase (5 U/μL) (1 X 100 μL)
● 10X T4 Ligation Buffer (1 X 200 μL)
● ATP Solution (25 mM) (1 X 80 μL)
● 50% PEG Solution (1 X 200 μL)
Technical Parameters
● Storage Temperature: -20°C
● One Weiss unit catalyzes the conversion of 1 nmol of 32P from
pyrophosphate into Norit-adsorbable material in 30 minutes at 37°C.
Applications
The Avam® T4 DNA Ligase Molecular Biology Grade Kit is ideal for various
molecular biology applications, including:
● Molecular cloning of PCR products or restriction fragments
● Site-directed mutagenesis
● Nick repair in duplex DNA, RNA, or DNA/RNA hybrids
● Self-circularization of linear DNA
● Ligation Mediated PCR (LM PCR) methods
Product FAQs
How do I thaw and resuspend the 10x Ligation Buffer and ATP Solution?
Thaw the 10x Ligation Buffer and ATP Solution at room temperature, and gently mix or vortex to ensure complete resuspension before use.
What type of ligations is T4 DNA Ligase suitable for?
T4 DNA Ligase is suitable for both blunt-end ligations and cohesive-end ligations, offering versatility in molecular cloning applications.
How can I improve electrotransformation efficiency when using Avam® T4 DNA Ligase Molecular Biology Grade?
Enhance electrotransformation efficiency by heat-inactivating the Avam® T4 DNA Ligase Molecular Biology Grade and purifying DNA through a spin column purification method.
What is the recommended ratio of insert DNA to vector DNA for ligation reactions?
We recommend using a 3-10 molar excess of insert DNA over vector DNA to optimize ligation efficiency.
How can I inactivate Avam® T4 DNA Ligase Molecular Biology Grade after ligation reactions?
Avam® T4 DNA Ligase Molecular Biology Grade can be inactivated by incubating at 65°C for 10 minutes or at 70°C for 5 minutes.
What are the conditions for optimal enzyme activity?
The enzyme is inhibited by concentrations of >200 mM NaCl or KCI. Optimal ligation conditions may vary depending on specific experimental requirements.
Can Avam® T4 DNA Ligase Molecular Biology Grade be used for site-directed mutagenesis?
Yes, Avam® T4 DNA Ligase Molecular Biology Grade can be used for site-directed mutagenesis, among other molecular cloning applications.
| Quantity | 2500U, 5000U, 500U |
|---|
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