Avam® T3 DNA Ligase is an efficient DNA ligase for the seamless joining of DNA fragments, suitable for various molecular biology applications.
Avam® T3 DNA Ligase
$470.00
Purchase this item and get Product Description
The Avam® T3 DNA Ligase offers a high-performance solution for DNA ligation, enabling the formation of phosphodiester bonds between DNA fragments with blunt-end and cohesive-end termini. This includes 900,000 U of Avam® T3 DNA Ligase (3,000,000 U/mL) and 2X Fast Ligation Buffer, providing optimal conditions for efficient ligation reactions. Avam® T3 DNA Ligase exhibits enhanced salt tolerance and can efficiently ligate DNA fragments with different overhangs, making it versatile for applications such as cloning ligation and nick-sealing in double-stranded DNA. With its high purity and specific activity, Avam® T3 DNA Ligase ensures reliable and reproducible results in molecular biology experiments.
Kit Contents
● 900,000 U of Avam® T3 DNA Ligase (3,000,000 U/mL)
● 2X Fast Ligation Buffer
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 37,350 Daltons
● Purity: >99%
● Specific activity: 3,000,000 U/mg
● Single-stranded exonuclease: <1% released
● Double-stranded exonuclease: <1% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
Applications
● Cloning ligation
● Nick-sealing in double-stranded DNA
Product FAQs
What is the recommended reaction time for ligation with Avam® T3 DNA Ligase?
The recommended incubation time for ligation reactions using Avam® T3 DNA Ligase is typically between 15 to 30 minutes at 25°C. Longer incubation times may be required for specific applications or reaction conditions.
Can Avam® T3 DNA Ligase be used for blunt-end ligation without PEG 6000?
While Avam® T3 DNA Ligase is capable of blunt-end ligation, its efficiency is significantly enhanced in the presence of PEG 6000. Without PEG 6000, the efficiency of blunt-end ligation may be low.
What is the optimal magnesium chloride (MgCl2) concentration for Avam® T3 DNA Ligase reactions?
The optimal magnesium chloride concentration for Avam® T3 DNA Ligase reactions is provided in the 2X Fast Ligation Buffer and is typically 20 mM. This concentration has been optimized to ensure efficient ligation performance.
Can Avam® T3 DNA Ligase be used for ligating DNA fragments with different overhangs?
Yes, Avam® T3 DNA Ligase can efficiently ligate DNA fragments with different overhangs, including A/T overhangs and cohesive ends. It is versatile and suitable for various ligation applications.
Is Avam® T3 DNA Ligase recommended for high-salt ligation reactions?
Yes, Avam® T3 DNA Ligase exhibits increased salt tolerance compared to other DNA ligases, making it suitable for ligation reactions with higher salt concentrations. It retains 95% of its activity in 1.0 M NaCl or KCI, with an optimal concentration of 300 mM.
| Quantity | 900000U |
|---|
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