The Avam® ssDNA Binding Protein binds single-stranded DNA, relaxes secondary DNA structure, and enhances processivity in certain DNA polymerase-mediated reactions for a hassle-free PCR experience.
Avam® Single Stranded DNA Binding Protein (E. coli)
$474.00
Purchase this item and get Product Description
Avam® ssDNA Binding Protein (E. coli) selectively binds single-stranded DNA, forming tetramers of four identical 18.9 kDa subunits, providing protection to 8-16 nucleotides. Primarily involved in DNA replication, recombination, and repair processes in nature, this protein enhances specific DNA polymerase-mediated reactions in vitro by relaxing DNA secondary structure and boosting enzyme processivity. Its ability to bind single-stranded DNA effectively allows for the stabilization of DNA regions, minimizing secondary structure formation and facilitating the fidelity of DNA polymerase during replication and amplification processes. Supplied in a buffer solution containing 50 mM Tris-HCl, 200 mM NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol, pH 7.5 at 25°C, this protein is a versatile tool for various molecular biology applications, aiding in the manipulation and analysis of DNA molecules with enhanced precision and efficiency.
Kit Contents
● Avam® ssDNA Binding Protein (E. coli) (1.5 mg)
● Storage buffer solution
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 18.975 Kilo Daltons
● Purity: >99%
● Single-stranded exonuclease: <1% released
● Double-stranded exonuclease: <1% released
● Double-stranded endonuclease: No conversion
● E.coli DNA contamination: <0.7 µg inhibits PCR
Applications
● Enhances DNA Polymerase Fidelity
● Facilitates primer sequestration
● Allows for longer read lengths in pyrosequencing for SNP analysis
Product FAQs
What is the role of Avam® ssDNA Binding Protein (E. coli) in DNA polymerase reactions?
This protein stabilizes single-stranded DNA regions, reduces DNA secondary structure, and enhances the fidelity and processivity of certain DNA polymerase-mediated reactions.
How should Avam® ssDNA Binding Protein (E. coli) be stored?
It should be stored at temperatures ranging from -25°C to -15°C for optimal stability.
What concentration of the protein is recommended for PCR reactions?
As a starting point, add Avam® ssDNA Binding Protein (E. coli) at a concentration range between 20-320 ng/µL per 50 µL reaction to identify the optimal concentration for PCR reactions.
How is the purity and activity of the protein assessed?
Purity and activity are evaluated through various quality control measures, including SDS-PAGE, specific activity assays, and tests for contaminating E. coli DNA.
Can this protein be used in applications other than DNA polymerase reactions?
Yes, it can also be utilized in primer sequestration and for enabling longer read lengths in pyrosequencing for SNP analysis.
| Quantity | 1.5mg |
|---|
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