Avam® RNase H MBG is a high-quality recombinant enzyme, suitable for molecular biology applications, available in a 5 U/mL concentration and stored at -20°C.
Avam® RNase H MBG
Price range: $93.10 through $376.00
Purchase this item and get Product Description
Avam® RNase H MBG is a recombinant endoribonuclease purified from Escherichia coli, overexpressing the cloned RNase H gene (rnh). With a specific activity of 5 U/mL, this enzyme specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA, producing 5′ phosphate-terminated oligoribonucleotides and single-stranded DNA. It prevents the degradation of single and double-stranded DNA or unhybridized RNA, making it essential for various molecular biology applications.
Supplied with a 10X Reaction Buffer containing 200 mM Tris-HCI (pH 8.4), 500 mM KCI, 50 mM MgCl2, and 200 mM DTT, RNase H MBG is ready-to-use for RNA removal after first-strand cDNA synthesis.
Kit Contents
● Avam® RNase H MBG enzyme (5 U/mL concentration)
● 10X Reaction Buffer
Technical Parameters
● Protein Purity: >90%
● RNase Contamination: None detected
● DNase Contamination: None detected
● Proteases Contamination: None detected
Applications
Avam® RNase H MBG finds applications in:
● Removal of RNA after first strand cDNA synthesis (RT-PCR and
qRT-PCR)
● Removal of mRNA prior to synthesis of second strand cDNA
● Removal of the poly(A) sequences of mRNA after hybridization with
oligo(dT)
● Site-specific cleavage of RNA
● Studies of in vitro polyadenylation reaction products
Product FAQs
What is the storage condition for Avam® RNase H MBG?
It should be stored at -20°C to maintain its stability and activity.
How should I prepare a reaction with Avam® RNase H MBG?
Use 5 U of the enzyme to remove RNA from an RNA:DNA duplex after reverse transcription in a 20 µL reaction. If a 50 µL reaction is desired, 12.5 U of the enzyme is recommended.
Can Avam® RNase H MBG be used for RT-PCR and qRT-PCR applications?
Yes, it is specifically designed for the removal of RNA after first-strand cDNA synthesis, making it suitable for RT-PCR and qRT-PCR workflows.
Does Avam® RNase H MBG exhibit any contamination with DNase, RNase, or proteases?
No, it is rigorously tested and confirmed to be free of DNase, RNase, and protease contamination.
| Quantity | 1250U, 250U |
|---|
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