Avam® RNase H

Avam® RNase H

$443.00

Avam® RNase H facilitates precise RNA degradation in RNA/DNA hybrid molecules, ensuring exceptional purity and specificity for reliable results in molecular biology applications such as RT-PCR, cDNA synthesis, and RT-qPCR.
Purchase this item and get 44 Points
Purchase this item and get 44 Points
Product Description

Avam® RNase H is an essential tool for researchers involved in molecular biology applications requiring accurate RNA degradation. This kit contains E. coli RNase H, an endoribonuclease that selectively cleaves the RNA strand of RNA/DNA hybrid molecules, leaving behind ribonucleotide molecules with 5′-phosphate and 3′-hydroxyl termini. With high purity and specificity, the Avam® RNase H enzyme ensures minimal off-target effects, leading to reliable and reproducible results in downstream assays.

Designed for convenience and efficiency, the Avam® RNase H includes 1 mL of RNase H enzyme solution (5000 U/mL) and 2 x 1.5 mL of 10X Avam® RNase H Buffer. The enzyme is purified from a recombinant E. coli strain carrying the RNase H (mh) gene from E. coli, guaranteeing high-quality performance in RNA degradation applications.

Ideal for RT-PCR, cDNA synthesis, and RT-qPCR, the Avam® RNase H offers researchers a versatile solution for various molecular biology experiments. Whether removing mRNA during second-strand cDNA synthesis or enhancing the sensitivity of RT-qPCR assays, this kit provides the precision and reliability needed for successful experimental outcomes.

Kit Contents

● Avam® RNase H (5000 U/mL) ( 1 X 1 mL)
● 10X Avam® RNase H Buffer (2 X 1.5 mL)

Technical Parameters

● Storage temperature: –25°C to –15°C
● Molecular weight: 18.1 kDa
● Purity: >99%
● Specific activity: 625,000 U/mg
● Single-stranded exonuclease: <5.0% released
● Double-stranded exonuclease: <1.0% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
● Non-specific RNase: No detectable non-specific RNase

Applications

● RT-PCR
● cDNA synthesis
● RT-qPCR

Product FAQs
Can Avam® RNase H be used for RNA degradation in RNA/DNA hybrid molecules of varying lengths?

Yes, Avam® RNase H enzyme is effective in degrading RNA/DNA hybrid molecules of different lengths, ensuring precise and reliable RNA degradation regardless of the target sequence’s complexity.

What quality control measures are implemented for Avam® RNase H?

Avam® RNase H undergoes rigorous quality control testing to ensure high purity, specificity, and performance. Quality control measures include protein purity assessment, DNase and RNase contamination testing, and evaluation of enzyme activity.

Can I use Avam® RNase H for RNA degradation in complex RNA templates?

Yes, Avam® RNase H enzyme possesses supreme thermostability and is suitable for complex RNA templates. It efficiently degrades RNA strands in RNA/DNA hybrid molecules, even in the presence of secondary structures or GC-rich regions.

Is there any risk of non-specific RNase activity with Avam® RNase H?

No, Avam® RNase H enzyme is rigorously tested for non-specific RNase contamination using sensitive assays. It demonstrates high specificity, ensuring targeted RNA degradation without non-specific cleavage events.

Quantity

5000U
SKU: EN8015 Category:
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