The Avam® RNase-Free DNase Set ensures efficient removal of DNA during RNA purification, enhancing the quality of RNA samples for sensitive downstream applications.
Avam® RNase Free DNase Set
Price range: $154.00 through $701.00
Purchase this item and get Product Description
The Avam® RNase-Free DNase Set is a reliable solution for achieving high-quality RNA samples free of contaminating DNA. Designed for efficient on-column digestion of DNA during RNA purification. While GeneEase® Kits typically remove the majority of DNA without the need for DNase treatment, certain RNA applications may require more thorough DNA removal. RNase Free DNase Digestion Buffer, included in the set, is specifically optimized for on-column DNase digestion, allowing for a quick and effective treatment in just 15 minutes at 20-30°C. Additionally, the buffer can be used for efficient DNase digestion in solution, offering versatility in experimental design.
The Avam® RNase-Free DNase Set comes with 1500 Kunitz units of RNase-free DNase I, ensuring reliable and consistent performance. Each Kunitz unit is defined as the amount of DNase I required to cause a specific increase in A260 per minute per milliliter under standardized conditions. This lyophilized enzyme is delivered stable and ready for use, providing researchers with the confidence to obtain high-quality RNA samples for sensitive downstream applications such as RNA-related molecular diagnostics, cDNA synthesis, RT-PCR, and more.
Kit Contents
● Avam® RNase-free DNase I (1500 Kunitz units)
● RNase-free DNase Digestion Buffer
● Nuclease-free water
Technical Parameters
● One Kunitz unit is defined as the amount of DNase I that causes an increase in A260 of 0.001 per minute per milliliter at 25°C, pH 5.0, with highly polymerized DNA as the substrate.
Applications
● Efficient on-column digestion of DNA during RNA purification
● Compatible with commercially available RNA isolation kits
● Ideal for sensitive downstream applications such as RNA-related
molecular diagnostics, cDNA synthesis, RT-PCR, and more
Product FAQs
What is the definition of one Kunitz unit?
One Kunitz unit is defined as the amount of DNase I required to cause an increase in A260 of 0.001 per minute per milliliter at 25°C, pH 5.0, with highly polymerized DNA as the substrate.
How long does the DNase digestion take with RNase Free DNase Digestion Buffer?
DNase digestion with RNase Free DNase Digestion Buffer typically takes 15 minutes at 20-30°C for efficient on-column DNA digestion during RNA purification.
Can this set be used with other RNA isolation kits?
While optimized for use with GeneEase™ procedures and the AvaGenix™ RNA Blood Mini Kit, this set can be adapted for use with other RNA isolation kits with appropriate protocol adjustments.
| Quantity | 250 preparations, 50 preparations |
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