Molecular biology grade RNase A (Ribonuclease A) supplied in a salt-free, freeze-dried form or in a 50% glycerol solution, suitable for various molecular biology applications.
Avam® RNase A MBG
Price range: $54.60 through $78.10
Purchase this item and get Product Description
Avam® RNase A (Molecular Biology Grade) is a high-purity endoribonuclease derived from bovine pancreas, specifically designed for molecular biology applications. With a monomeric weight of 13.7 kDa, this enzyme selectively cleaves single-stranded RNA 3′ next to pyrimidine residues, such as cytosine and uracil, degrading RNA into cyclic nucleotide monophosphates to 5′-OH and 2′-, 3′-cyclic monophosphate. It possesses enzyme activity of over 80 units/mg protein and exhibits no endonuclease or exonuclease activity towards DNA substrates.
Supplied in a salt-free, freeze-dried form or in a 50% glycerol solution, Avam® RNase A MBG is versatile and can be stored at -20°C for long-term storage or at 4°C for up to several weeks. Each unit of activity is defined as the amount of enzyme that causes the hydrolysis of RNA to yield a velocity constant (k=1) at 25°C and pH 5.0.
Kit Contents
● Avam® RNase A MBG (10mg/50mg)
Technical Parameters
● Activity: 94.5 U/mg (Kunitz)
● DNase Contamination: None detected
● Protease Contamination: None detected
Applications
Avam® RNase A MBG finds applications in:
● Purification of RNA-free DNA
● Isolation of plasmid and genomic DNA
● Removal of RNA during recombinant protein preparations
● Protection of RNA during assays
● Mapping of single-based mutations in DNA or RNA
Product FAQs
What is the storage condition for Avam® RNase A MBG?
It can be stored at -20°C for long-term storage in a lyophilized or glycerol solution form or at 4°C for short-term storage.
How should I prepare a working solution of Avam® RNase A MBG?
Resuspend the enzyme in 10 mM Tris-HCI (pH 7.5), 15 mM NaCl, 50% (v/v) glycerol, or TE buffer to a final concentration of 1-10 mg/mL.
Can Avam® RNase A MBG be used for RNA removal from plasmid preparations?
Yes, it is recommended to use a 10 µg/mL working solution and incubate the sample for 1 hour at room temperature.
Does Avam® RNase A MBG exhibit any DNase or protease contamination?
No, it is tested and confirmed to be free of DNase and protease contamination.
| Quantity | 10mg, 50mg |
|---|
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