Avam® RNase A Inhibitor is a highly effective inhibitor of pancreatic-type ribonucleases A, B, and C, available in a concentrated form of 20,000 U. It ensures RNA integrity in molecular biology experiments.
Avam® RNase A Inhibitor
$474.00
Purchase this item and get Product Description
Avam® RNase A Inhibitor is an indispensable tool for safeguarding RNA integrity in molecular biology applications. With its potent inhibitory action against pancreatic-type ribonucleases A, B, and C, this recombinant acidic protein, weighing 52 kDa, offers robust protection against RNA degradation. It is specifically designed as a non-competitive inhibitor, allowing it to bind with high affinity to targeted RNases without affecting other modifying enzymes like polymerases and reverse transcriptases. Supplied in a stable solution containing 20 mM Hepes-KOH, 50 mM KCI, 8 mM DTT, and 50% glycerol, with a pH of 7.5 at 25°C, this RNase Inhibitor ensures RNA stability throughout experimental procedures.
Kit Contents
● Avam® RNase A Inhibitor enzyme (20,000 U concentration)
Technical Parameters
● Purity: >99%
● Specific Activity: 53,333 U/mg
● Single-stranded exonuclease: <5.0% released
● Double-stranded exonuclease: <1.0% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
● RNase contamination: No detectable non-specific RNase
Applications
Avam® RNase A Inhibitor is essential for applications where RNase presence
may affect RNA quality and experimental results, including:
● RNA isolation and purification
● cDNA synthesis
● RT-PCR and RT-qPCR
● In vitro transcription and translation
● RNase-free monoclonal antibody production
Product FAQs
What is the storage temperature for the Avam® RNase A Inhibitor?
It should be stored at -25°C to -15°C for long-term stability and activity.
How does the Avam® RNase A Inhibitor work?
It inhibits pancreatic-type ribonucleases A, B, and C by binding non-covalently with high affinity in a 1:1 ratio, effectively preventing RNA degradation.
Can the Avam® RNase A Inhibitor be used with other modifying enzymes?
Yes, it does not interfere with the activities of other RNases or modifying enzymes like polymerases and reverse transcriptases.
What are the quality control measures for the Avam® RNase A Inhibitor?
Unit activity, protein concentration, physical purity, single-stranded exonuclease, double-stranded exonuclease, double-stranded endonuclease, and E. coli contamination are rigorously evaluated to ensure high quality and performance consistency.
| Quantity | 20000U |
|---|
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