Enhance nucleic acid isolation with the Avam® Proteinase K, available in both powder and solution forms, as well as an ultrapure variant. Designed for molecular biology applications, this kit offers exceptional purity and activity, ensuring reliable and reproducible results.
Avam® Proteinase K
Price range: $0.00 through $2,567.00
Purchase this item and get Product Description
The Avam® Proteinase K comprises Proteinase K enzyme in powder or solution form, along with an ultrapure variant, and is tailored for various molecular biology applications. Derived from Parengyodontium album (Tritirachium album), Proteinase K is a broad-spectrum serine protease renowned for its robust activity across a wide range of reaction conditions, including elevated temperatures and denaturing environments. It effectively digests proteins, DNases, and RNases during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. The enzyme, expressed in Komagataella phaffii (Pichia pastoris), undergoes extensive purification to yield high-quality products with reduced DNA content, ensuring minimal cross-contamination in next-generation sequencing (NGS) and diagnostic applications. The kit offers flexibility with options for liquid or lyophilized formats, catering to diverse experimental requirements.
Kit Contents
Available as Proteinase K Powder or Solution or Ultrapure powder in a variety of pack sizes.
Technical Parameters
● Molecular Biology Grade: Liquid or White lyophilized powder
● Ultrapure Grade: White lyophilized powder
● Storage: -20°C
● Protein content: >70%
● Solubility in water: ≥20 mg/mL (Powder), ≥50 mg/mL (Ultrapure)
● Activity: ≥30 U/mg (Powder and Solution), ≥35 U/mg (Ultrapure)
● DNA content: ≤10 pg/mg (Powder and Solution), ≤0.1 pg/mg (Ultrapure)
● Single-stranded exonuclease: Negative
● Double-stranded exonuclease: Negative
● Double-stranded endonuclease: Negative
Applications
● DNA and RNA extraction from various sample types
● DNase and RNase elimination during nucleic acid isolation
● Purification of protein-contaminated samples
● Automation of isolation processes
Product FAQs
What is the recommended storage temperature for the Avam® Proteinase K?
The kit should be stored at -20°C to maintain enzyme stability and activity.
What are the advantages of using Avam® Proteinase K Ultrapure?
Avam® Proteinase K Ultrapure offers increased solubility, activity, and purity, with minimal DNA contamination, making it suitable for sensitive molecular biology applications.
Can Avam® Proteinase K be used for automated isolation processes?
Yes, the Avam® Proteinase K is compatible with automation and can be integrated into nucleic acid isolation workflows for increased efficiency and throughput.
| Powder / Solution options | Avam® Proteinase K Powder, Avam® Proteinase K Solution, Avam® UltraPure Proteinase K Powder |
|---|---|
| Product Quantity | 20 mg, 100 mg, 250 mg, 1 g, 10 g, 25 g, 50 g, 100 g, 1 mL, 5 mL, 10 mL, 100 mL, 500 mL |
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