Achieve efficient protease digestion in DNA and RNA isolation procedures with our Avam® Protease and Proteinase K Kit, offering high activity and compatibility with selected Avam® protocols.
Avam® Protease and Proteinase K
Price range: $50.40 through $365.00
Purchase this item and get Product Description
The Avam® Protease and Proteinase K provides essential enzymes for efficient protease digestion in DNA and RNA isolation procedures. Avam® Protease, a recombinant serine protease isolated from a Bacillus strain, offers stability and high activity, particularly in alkaline pH conditions (pH 7.5 – 10.5). It exhibits 30 to 40% higher specific activity than Proteinase K in Tris-containing buffers of alkaline pH. Additionally, Avam® Protease demonstrates comparable activity to Proteinase K in the presence of strong denaturants like urea and guanidine HCl, with an EDTA concentration of less than 8 mM. The enzyme can be efficiently inactivated by incubation at 70°C for 15 minutes, making it suitable for various molecular biology applications. Proteinase K, derived from the saprophytic fungus Tritirachium album, offers broad substrate specificity and stability over a wide range of temperatures and pH values. It possesses high specific activity, which remains stable even at higher temperatures, and does not require soluble calcium for enzymatic activity. This unique feature makes Avam® Proteinase K ideal for short digestion times and ensures reliable performance in DNA and RNA isolation protocols.
Both enzymes included in the kit are quality-guaranteed by Avam®, ensuring consistent and reproducible results. With their compatibility with selected Avam® protocols and versatility in various molecular biology applications, the Avam® Protease and Proteinase K Kit serve as indispensable tools for researchers engaged in DNA and RNA preparation and analysis.
Kit Contents
Avam® Protease (lyophilized powder)
Avam® Proteinase K (ready-to-use solution)
Avam® Protease Solvent
Technical Parameters
● Format: Lyophilized powder (Avam® Protease), Ready-to-use solution (Avam® Proteinase K)
● Activity: Avam® Protease – 7.5 Anson units per vial (lyophilized), Avam® Proteinase K – >600 mAU/ml
● Unit definition: One mAU is the activity that releases folin-positive amino acids and peptides corresponding to 1 μmol tyrosine per minute
Applications
The Avam® Protease and Proteinase K Kit are essential tools for protease digestion during DNA and RNA preparation. They are particularly suitable for use with selected Avam® protocols and find applications in various molecular biology procedures.
Product FAQs
What is the activity of Avam® Protease and Proteinase K?
Avam® Protease exhibits 7.5 Anson units per vial (lyophilized), while Proteinase K has an activity of >600 mAU/ml.
Are these enzymes compatible with selected Avam® protocols?
Yes, both Avam® Protease and Proteinase K are fully compatible with selected Avam® protocols for DNA and RNA isolation.
What is the storage condition for Avam® Protease Solvent?
Avam® Protease Solvent should be stored according to the manufacturer’s recommendations provided in the kit manual.
Can Avam® Protease be used in the presence of strong denaturants?
Yes, Avam® Protease is stable and active in the presence of denaturants such as urea and guanidine HCl, provided the EDTA concentration is less than 8 mM.
| Enzyme / Solution type | Avam® Protease, Avam® Proteinase K, Avam® Protease Solvent |
|---|---|
| Product Quantity | 7.5 AU, (4 X 7.5) AU, 2mL, N/A |
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