Obtain high-quality plasmid DNA with ease using Avam® Plasmid Kits, offering purity equivalent to 2 x CsCl gradient centrifugation, high yields, and cost-effective preparations.
Avam® Plasmid Kits
Price range: $174.00 through $4,671.00
Purchase this item and get Product Description
Avam® Plasmid Kits revolutionize plasmid DNA purification, delivering superior quality DNA with minimal effort. These kits utilize gravity-flow, anion-exchange tips to isolate transfection-grade plasmid DNA efficiently. Featuring a unique integrated ATP-dependent exonuclease digestion step, these kits ensure the selective removal of contaminating genomic DNA, resulting in unparalleled purity. Additionally, the inclusion of IndigoLyse enhances lysis efficiency, maximizing DNA yield. With a streamlined and user-friendly protocol, these kits offer a convenient solution for plasmid DNA purification needs across various applications. Whether you’re conducting transfection experiments, cloning, or sequencing, trust Avam® Plasmid Kits for consistent and reliable results.
Kit Contents
Each kit contains Avam® Flow Tips, reagents, buffers, and VividBlue Buffer for optimal lysis. The specific contents vary depending on the kit size and format.
Technical Parameters
● Culture Volume/Starting Material: Ranges from 3 ml to 5 liters
depending on the kit size
● Elution Volume: Variable
● Plasmid Type: High-copy, low-copy, cosmid DNA
● Processing: Manual (gravity flow)
● Sample per Run: 1 sample per run
● Technology: Anion-exchange technology
● Time per Run: Ranges from 80 minutes to 320 minutes depending on the kit size
● Yield: Ranges from <20 µg to <10 mg depending on the kit size
Applications
Plasmid DNA purified using the Avam® Plasmid Kits is suitable for various applications, including transfection, cloning, PCR, and in vitro transcription.
Product FAQs
What are the advantages of using the Avam® Plasmid Kits?
The Avam® Plasmid Kits offer purity equivalent to 2X CSCI gradient centrifugation, high yields of plasmid DNA, and cost-effective preparations.
Can these kits be used for the purification of plasmid DNA from different culture volumes?
Yes, the kits are available in various formats suitable for purification from different culture volumes, ranging from 3 ml to 5 liters
How long does it take to perform plasmid purification using these kits?
The time required for plasmid purification varies depending on the kit size, ranging from 80 minutes to 320 minutes.
What downstream applications are suitable for the plasmid DNA purified using these kits?
Plasmid DNA purified with the Avam® Plasmid Kits is suitable for transfection, cloning, PCR, and in vitro transcription.
Can these kits be used for purification of both high-copy and low-copy plasmids?
Yes, the Avam® Plasmid Kits are suitable for purification of various types
of plasmids, including high-copy, low-copy, and cosmid DNA.
| Quantity | Avam AnioFlow Tips: 25 units, Avam Filter Cartridges with filter buffer: 5 units, Buffer Kit, Giga Plasmid Kit: 5 units, Maxi Plasmid Kit: 10 units, Maxi Plasmid Kit: 100 units, Maxi Plasmid Kit: 25 units, Mega Plasmid Kit: 25 units, Mega Plasmid Kit: 5 units, Midi Plasmid Kit: 100 units, Midi Plasmid Kit: 25 units, Midi Plasmid Kit: 400 units, Mini Plasmid Kit: 100 units, Mini Plasmid Kit: 25 units |
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