The Avam® Klenow Fragment is a revolutionary solution for precise DNA synthesis and proofreading in molecular biology applications. With its exceptional strand displacement and moderate proofreading activity, this kit ensures accurate DNA synthesis with minimal error rates.
Avam® Klenow Fragment
$470.00
Purchase this item and get Product Description
The Avam® Klenow Fragment stands as an indispensable tool in molecular biology, offering unparalleled precision and versatility in DNA synthesis and proofreading. Derived from the DNA-dependent repair enzyme of Escherichia coli, this kit presents researchers with a robust solution for a wide array of applications, ranging from routine cloning to sophisticated genetic engineering endeavors.
At the heart of this kit les the Klenow Fragment, a mesophilic DNA polymerase renowned for its exceptional performance in DNA synthesis. This polymerase, lacking 5′→3′ exonuclease activity while retaining 3′→5′ exonuclease activity, facilitates accurate DNA replication with minimal error rates. Its moderate strand displacement activity further enhances its utility, allowing for efficient synthesis of DNA fragments and filling of gaps in nucleatide sequences.
The Avam® Klenow Fragment Kit includes 2500 units of Klenow Fragment, meticulously prepared at a concentration of 5000 U/mL, ensuring optimal enzymatic activity for various experimental setups. Additionally, the kit is complemented by 10x VividYellow™ Buffer, providing the necessary components for optimal enzyme function, induding salts, cofactors, and reducing agentis.
With its superior performance and reliability, the Avam® Klenow Fragment Kit empowers researchers to tackle a myriad of molecular biology challenges with confidence. Whether performing routine cloning procedures, tailing for next-generation sequencing, or engaging in intricate DNA labeling experiments, this kit serves as a cornerstone for achieving precise and reproducible results in the laboratory.
Unlock the full potential of your molecular biology endeavors with the Avam® Klenow Fragment Kita trusted companion for researchers seeking excellence in DNA synthesis and proofreading.
Kit Contents
● 50,000 U Avam® Exonuclease III (100,000 U/mL) (0.5 mL)
● 10X VividYellow™ Buffer (1 x 1.5 mL)
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 68.2 Kilo Daltons
● Purity: >99%
● Specific activity: 5000 U/mg
● Single-stranded exonuclease: 50 U; functional
● Double-stranded exonuclease: 50 U; functional
● Double-stranded endonuclease: 50 U; no conversion
● E. coli DNA contamination: 50 U; <10 copies
Applications
● Tailing for next-generation sequencing
● Strand displacement amplification
● DNA labeling
● Removal of 3’ overhangs and filling in of 5’ overhangs
Product FAQs
What is the storage temperature for the Avam® Klenow Fragment Kit?
The recommended storage temperature is between -25°C to -15°C.
Does the kit contain any DNA contamination?
The kit is tested to contain E. coli DNA contamination of less than 10 copies per 50 U.
Can the Avam® Klenow Fragment be used for DNA labeling?
Yes, the Avam® Klenow Fragment is suitable for DNA labeling applications.
Is strand displacement amplification possible with this kit?
Yes, the Avam® Klenow Fragmen kit enables strand displacement amplification techniques.
| Quantity | 2500U |
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