The Avam® HL-dsDNase is a highly efficient heat-labile dsDNase for targeted digestion of double-stranded DNA while preserving single-stranded DNA and RNA integrity.
Avam® HL-dsDNase
Price range: $433.00 through $2,137.00
Purchase this item and get Product Description
The Avam® HL-dsDNase is a highly efficient enzymatic solution designed for targeted digestion of double-stranded DNA (dsDNA) while preserving the integrity of single-stranded DNA (ssDNA) and RNA molecules, making it an invaluable tool for various molecular biology applications. Derived from a cold water eukaryotic organism and expressed in Pichia pastoris, this recombinant endonuclease exhibits exceptional specificity and activity over a broad temperature range of 10°C to 47°C.
With its unique property of yielding low-temperature activity, the Avam® HL-dsDNase offers a safeguarding mechanism to protect RNA or proteins from potential degradation during enzymatic digestion processes. It remains highly active in typical buffer formulations and exhibits robust performance across a wide pH range, with optimum activity observed at pH 7.0-8.0. Requiring at least 2 mM Mg2+ ions for optimal activity, this enzyme demonstrates irreversible inactivation at low temperatures, with a simple heat treatment at 52°C for 15 minutes in the presence of 1 mM DTT. This feature enables rapid and safe purification of RNA or protein samples from contaminating DNA, ensuring the integrity of downstream analyses.
Supplied with a buffer composition of 20 mM Tris-HCl, pH 8.0, 20 mM NaCl; 2 mM MgCl2: 1 mM CaCl2, and 50% (w/v) glycerol, the Avam® HL-dsDNase is conveniently formulated for immediate use in various experimental setups. This versatile enzymatic tool finds applications in a wide range of molecular biology workflows, including dsDNA digestion for plasmid and genomic DNA rapid purification of RNA and protein samples, decontamination of PCR and qPCR master mixes, and degradation of DNA templates in transcription reactions. Its robust performance and ease of use make it an indispensable asset in various laboratory settings, ensuring reliable and reproducible results in molecular biology experiments.
Kit Contents
● Avam® HL-dsDNase enzyme
● Buffer solution containing 20 mM Tris-HCl, pH 8.0; 20 mM NaCl; 2 mM MgCl2; 1 mM CaCl2; 50% glycerol
Technical Parameters
● Concentration: ≥2 U/μL
● Shelf life: 36 months
● Storage temperature: -20°C (long-term storage at -80°C, it is recommended that multiple freeze-thaw cycles be avoided)
Applications
Optimal for the following applications:
● Digestion of dsDNA in plasmid and genomic DNA samples
● Rapid purification of RNA and protein samples
● Decontamination of PCR and qPCR master mixes
● Degradation of DNA templates in transcription reactions
Product FAQs
What is the optimal temperature range for Avam® HL-dsDNase activity?
The Avam® HL-dsDNase exhibits high activity in a broad temperature range from 10°C to 47°C.
How can I inactivate Avam® HL-dsDNase?
The enzyme can be irreversibly inactivated by heat treatment at 52°C for 15 minutes in the presence of 1 mM DTT.
Can Avam® HL-dsDNase be used for RNA purification?
Yes, Avam® HL-dsDNase is suitable for rapid purification of RNA samples by selectively digesting dsDNA contaminants while preserving RNA integrity.
What is the storage stability of Avam® HL-dsDNase?
The enzyme has a shelf life of 36 months when stored at -20°C without a defrost cycle. For long-term storage, it is recommended to store at -80°C.
| Quantity | 2500U, 500U |
|---|
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