Avam® Exonuclease III

Product Description

Avam® Exonuclease III is a multifunctional enzyme crucial for various molecular biology applications, renowned for its precise excision of bases from duplex DNA in the 3′-5′ direction. Avam® Exonuclease III exhibits a diverse range of activities, including phosphatase, exonuclease, AP endonuclease, and RNase H activities.

This high-performance enzyme functions by selectively degrading one strand of a dsDNA duplex or the RNA strand of an RNA-DNA heteroduplex, facilitating precise and controlled deletions within the DNA molecule population. By efficiently removing 3 terminal groups on dsDNA and degrading 3 recessed ends. Avam® Exonuclease III generates 5′ overhangs, effectively breaking phosphodiester bonds on the 5′ side of AP sites in both double-stranded and single-stranded DNA. Additionally, Avam® Exonuclease III enhances the turnover of the DNA glycosylase Muty, further contributing to its multifaceted functionality.

Supplied in an optimized buffer solution, Avam® Exonuclease III offers exceptional stability and activity across a wide range of experimental conditions. The enzyme’s robust performance is further complemented by its compatibility with the provided 10X Vivid Yellow Buffer, ensuring consistent and reliable results.

Avam® Exonuclease III undergoes stringent quality control measures to ensure optimal purity and performance. Rigorous testing confirms its superior quality, with purity levels exceeding 99% and specific activity reaching 100,000 U/mg.

With its diverse capabilities, Avam® Exonuclease III is indispensable for a multitude of molecular biology applications. From DNA-protein interaction analysis and production of nested deletions to enzymatic homology-based DNA assembly and site-directed mutagenesis, this enzyme enables researchers to achieve precise and efficient DNA manipulation for a wide range of experimental needs. Whether preparing single-stranded DNA for dideoxy sequencing or generating strand-specific probes, Avam® Exonuclease III provides researchers with a powerful tool to streamline their molecular biology workflows and accelerate scientific discovery.

Kit Contents

● 50,000 U Avam® Exonuclease III (100,000 U/mL) (0.5 mL)
● 10X VividYellow™ Buffer (1 x 1.5 mL)

Technical Parameters

● Storage temperature: –25°C to –15°C
● Molecular weight: 98 Kilo Daltons
● Specific activity: 100,000 U/mg
● Double-stranded endonuclease activity: 1000 U = No conversion
● E. coli DNA contamination: <10 copies
● UDG activity: <20 U/mL
● Inactivation: Heating at 65°C for 5 minutes

Applications

● DNA-protein interaction analysis by Exo III DNA footprinting
● Production of nested deletions in double-stranded DNA
● Enzymatic homology-based DNA assembly via SENAX (Stellar
ExoNuclease Assembly mix)
● Cloning of PCR products
● Preparation of single-stranded DNA for dideoxy sequencing
● Site-directed mutagenesis
● Preparation of strand-specific probes

Product FAQs

What is the storage temperature for the Avam® Exonuclease III Kit?

The Avam® Exonuclease III Kit should be stored at temperatures ranging from -25°C to -15°C for optimal stability and performance.

Can the enzyme in the kit be heat-inactivated?

Yes, the 3′-5′ exonuclease activity of Avam® Exonuclease III can be efficiently inactivated by heating at 65°C for 5 minutes.

Is the Avam® Exonuclease III Kit compatible with various molecular biology buffers?

Yes, Avam® Exonuclease III works well in most molecular biology buffers containing magnesium over 1.5 mM, providing flexibility in experimental conditions.

What quality control measures are implemented for the Avam® Exonuclease lll Kit?

The Avam® Exonuclease III Kit undergoes rigorous quality control testing, including assessment of purity, specific activity, double-stranded endonuclease activity, E. coli DNA contamination, and UDG activity, ensuring superior quality and reliability for researchers.