Avam® Exonuclease III

$470.00

The Avam® Exonuclease III Kit offers researchers a flexible solution for the precise excision of bases from duplex DNA and targeted molecular biology manipulations. With its multifunctional enzyme exhibiting phosphatase, exonuclease, AP endonuclease, and RNase H activities, this kit enables controlled deletions within DNA molecules, facilitating various applications with exceptional reliability and reproducibility.
Purchase this item and get 47 Points
Purchase this item and get 47 Points
Product Description

Avam® Exonuclease III is a multifunctional enzyme crucial for various molecular biology applications, renowned for its precise excision of bases from duplex DNA in the 3′-5′ direction. Avam® Exonuclease III exhibits a diverse range of activities, including phosphatase, exonuclease, AP endonuclease, and RNase H activities.

This high-performance enzyme functions by selectively degrading one strand of a dsDNA duplex or the RNA strand of an RNA-DNA heteroduplex, facilitating precise and controlled deletions within the DNA molecule population. By efficiently removing 3 terminal groups on dsDNA and degrading 3 recessed ends. Avam® Exonuclease III generates 5′ overhangs, effectively breaking phosphodiester bonds on the 5′ side of AP sites in both double-stranded and single-stranded DNA. Additionally, Avam® Exonuclease III enhances the turnover of the DNA glycosylase Muty, further contributing to its multifaceted functionality.

Supplied in an optimized buffer solution, Avam® Exonuclease III offers exceptional stability and activity across a wide range of experimental conditions. The enzyme’s robust performance is further complemented by its compatibility with the provided 10X Vivid Yellow Buffer, ensuring consistent and reliable results.

Avam® Exonuclease III undergoes stringent quality control measures to ensure optimal purity and performance. Rigorous testing confirms its superior quality, with purity levels exceeding 99% and specific activity reaching 100,000 U/mg.

With its diverse capabilities, Avam® Exonuclease III is indispensable for a multitude of molecular biology applications. From DNA-protein interaction analysis and production of nested deletions to enzymatic homology-based DNA assembly and site-directed mutagenesis, this enzyme enables researchers to achieve precise and efficient DNA manipulation for a wide range of experimental needs. Whether preparing single-stranded DNA for dideoxy sequencing or generating strand-specific probes, Avam® Exonuclease III provides researchers with a powerful tool to streamline their molecular biology workflows and accelerate scientific discovery.

Kit Contents

● 50,000 U Avam® Exonuclease III (100,000 U/mL) (0.5 mL)
● 10X VividYellow™ Buffer (1 x 1.5 mL)

Technical Parameters

● Storage temperature: –25°C to –15°C
● Molecular weight: 98 Kilo Daltons
● Specific activity: 100,000 U/mg
● Double-stranded endonuclease activity: 1000 U = No conversion
E. coli DNA contamination: <10 copies
● UDG activity: <20 U/mL
● Inactivation: Heating at 65°C for 5 minutes

Applications

● DNA-protein interaction analysis by Exo III DNA footprinting
● Production of nested deletions in double-stranded DNA
● Enzymatic homology-based DNA assembly via SENAX (Stellar
ExoNuclease Assembly mix)
● Cloning of PCR products
● Preparation of single-stranded DNA for dideoxy sequencing
● Site-directed mutagenesis
● Preparation of strand-specific probes

Product FAQs
What is the storage temperature for the Avam® Exonuclease III Kit?

The Avam® Exonuclease III Kit should be stored at temperatures ranging from -25°C to -15°C for optimal stability and performance.

Can the enzyme in the kit be heat-inactivated?

Yes, the 3′-5′ exonuclease activity of Avam® Exonuclease III can be efficiently inactivated by heating at 65°C for 5 minutes.

Is the Avam® Exonuclease III Kit compatible with various molecular biology buffers?

Yes, Avam® Exonuclease III works well in most molecular biology buffers containing magnesium over 1.5 mM, providing flexibility in experimental conditions.

What quality control measures are implemented for the Avam® Exonuclease lll Kit?

The Avam® Exonuclease III Kit undergoes rigorous quality control testing, including assessment of purity, specific activity, double-stranded endonuclease activity, E. coli DNA contamination, and UDG activity, ensuring superior quality and reliability for researchers.

Quantity

50000U

SKU: EN1874 Category:
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