Avam® Exonuclease I

Avam® Exonuclease I

$470.00

The Avam® Exonuclease I is a highly efficient enzyme for removing single-stranded DNA from PCR products and plasmid prep cleanup.
Purchase this item and get 47 Points
Purchase this item and get 47 Points
Product Description

Avam® Exonuclease I is a versatile enzyme and has an exceptional ability to excise bases on single-stranded DNA in the 3′-5′ direction. This enzymatic activity, coupled with its 5′-3′ exonuclease function, enables the precise removal of linear single-stranded DNA while leaving the double-stranded DNA molecules and the 5′ terminus unaffected.

This kit offers a comprehensive solution for various DNA cleanup and processing protocols, facilitating seamless molecular biology workflows. By effectively removing unwanted single-stranded DNA fragments, Avam® Exonuclease I ensures the integrity and purity of DNA samples, thereby enhancing the reliability and accuracy of downstream applications.

The enzyme is highly processive, although digestion can be hindered by a 3-terminal phosphate. Avam® Exonuclease I is compatible with a broad range of buffer conditions and is optimally active in the presence of magnesium ions, typically added to reactions at concentrations ranging from 1 to 3 mM. Each component of the kit undergoes rigorous quality control measures to guarantee optimal performance and reliability. The enzyme is produced by a specialized strain of E. coli expressing the recombinant Exonuclease I gene ensuring high purity and activity.

In addition to its main applications in PCR product cleanup and plasmid prep, Avam® Exonuclease I finds utility in various molecular biology techniques, including the removal of single-stranded oligonucleotide primers, enhancing the accuracy and efficiency of DNA sequencing, cloning, and other downstream analyses.

With its superior performance and versatility, Avam® Exonuclease I is an indispensable tool for researchers seeking to achieve precise and reliable DNA cleanup and processing in their experiments.

Kit Contents

● Avam® Exonuclease I Enzyme (1X 1.5mL)

Technical Parameters

● Storage temperature: –25°C to –15°C
● Molecular weight: 54.5 Kilo Daltons
● Purity: >99%
● Specific activity: 185,000 U/mg
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies

Applications

● Plasmid prep cleanup
● Removal of single-stranded DNA from PCR products
● Cleanup of oligonucleotide primers after PCR

Product FAQs
What is the principle behind Avam® Exonuclease I's activity?

Avam® Exonuclease I cleaves single-stranded DNA in the 3′-5′ direction, releasing 5 monad-nucleotides and leaving double-stranded DNA molecules and the 5′ terminus intact.

How should I use Avam® Exonuclease I in my experiments?

For removal of single-stranded DNA from PCR products, set up the reaction mixture and incubate at 37°C for 15 minutes. Heat inactivate the enzyme at 80°C for 15 minutes.

What are the specific applications of Avam® Exonuclease I?

Avam® Exonuclease I is commonly used for plasmid prep cleanup, removal of single-stranded DNA from PCR products, and cleanup of oligonucleotide primers after PCR.

Quantity

30000U
SKU: EN6032 Category:
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