Robust enzymatic solution for excising damaged pyrimidines from DNA and cleaving AP sites, leaving terminal phosphates at both 5ʹ and 3ʹ ends.
Avam® Endonuclease VIII
$474.00
Purchase this item and get Product Description
Avam® Endonuclease VIII from E. coli functions as an N-glycosylase by excising oxidative base lesions, generating AP sites, and subsequently acting as an AP-lyase, cleaving the phosphodiester backbone. It effectively removes various damaged bases such as urea, 6,6-dihydroxy thymine, and thymine glycol. The enzyme is supplied in a buffer optimized for its activity, facilitating its usage in molecular biology applications. Avam® Endonuclease VIII is an essential tool for DNA repair studies and is particularly valuable in investigating oxidative DNA damage mechanisms and assessing DNA repair processes. Its ability to excise damaged pyrimidines and cleave AP sites with high efficiency makes it indispensable in various research areas, including genomics, DNA damage and repair, and environmental toxicology.
Kit Contents
● 10,000 U of Avam® Endonuclease VIII (1 X 1 mL)
● 10X Avam® Endonuclease VIII Buffer (1 X 1.5 mL)
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 29.845 Kilo Daltons
● Purity: >99%
● Specific activity: 770,000 U/mg
● Single-stranded exonuclease: <1% released
● Double-stranded endonuclease: <1% released
● E. coli DNA contamination: <10 copies
Applications
● Excision of damaged pyrimidines from duplex DNA
● Cleaving AP sites, leaving terminal phosphates at both 5ʹ and 3ʹ ends
● Measuring DNA damage through single cell gel electrophoresis or comet assay
Product FAQs
What is the principle behind Avam® Endonuclease VIII's activity?
Avam® Endonuclease VIII acts as both an N-glycosylase and an AP-lyase, excising damaged bases and cleaving the phosphodiester backbone, respectively.
How should I use Avam® Endonuclease VIII in my experiments?
Add Avam® Endonuclease VIII to your DNA substrate reaction and incubate at 37°C for 30 minutes. Heat inactivation can be carried out at 75°C for 10 minutes.
How is the quality of Avam® Endonuclease VIII ensured?
Avam® Endonuclease VIII undergoes rigorous quality control measures, including specific activity assays and assessment of physical purity by SDS-PAGE and silver-stain detection.
What are the specific applications of Avam® Endonuclease VIII?
Avam® Endonuclease VIII is commonly used for excising damaged pyrimidines from DNA, cleaving AP sites, and measuring DNA damage in various assays such as single-cell gel electrophoresis or comet assay.
| Quantity | 10000U |
|---|
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