Avam® dsDNase offers an efficient solution for targeted degradation of double-stranded DNA, preserving single-stranded DNA and RNA integrity in molecular biology applications.
Avam® dsDNase
$134.00
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Avam® dsDNase offers a powerful solution for targeted degradation of double-stranded DNA, while preserving the integrity of single-stranded DNA and RNA molecules in molecular biology experiments. Derived from marine amphipods and expressed in Pichia pastoris, this recombinant enzyme exhibits high specificity and efficiency in cleaving double-stranded DNA into fragments below 10 nucleotides.
With its broad activity spectrum encompassing temperatures from 10°C to 80°C and pH levels ranging from 6.0 to 9.0, the dsDNase enzyme demonstrates robust performance across diverse experimental conditions. Its remarkable stability and activity, even in the presence of elevated salt concentrations and common buffer additives, make it an indispensable tool for various applications.
Utilizing bivalent cations such as Mg2+ and Ca2+ for maximum activity, this enzyme excels in selectively degrading double-stranded DNA, with activity levels towards dsDNA at least 1000 times higher than towards single-stranded DNA. The resulting cleavage products retain a 5′-phosphate and 3′-hydroxyl group configuration, facilitating downstream analysis without compromising the integrity of RNA or single-stranded DNA templates.
Whether used for RNA extraction and purification, removal of genomic DNA contamination from RNA samples, or degradation of DNA templates in transcription reactions, the Avam dsDNase Kit delivers consistent and reliable performance. Additionally, it aids in reducing sample viscosity and eliminating residual DNA during primary stem cell isolation, biopharmaceutical production, and bioprocessing procedures.
Overall, the Avam® dsDNase empowers researchers with a versatile and efficient tool for precise manipulation of nucleic acids, ensuring the purity and integrity required for accurate molecular biology analyses and downstream applications
Kit Contents
● Avam® dsDNase enzyme (5000 U)
● 20 mM Tris-HCl, pH 8.0
● 50 mM KCl
● 5 mM MgCl2
● 50% (v/v) glycerol
Technical Parameters
● Concentration: ≥20 U/µL
● Shelf life: 36 months
● Storage temperature: -20°C (It is recommended that multiple freeze-thaw cycles be avoided)
Applications
Optimal for the following applications:
● RNA extraction and purification
● Removal of contaminating genomic DNA from RNA samples
● DNA template degradation in transcription reactions
● Reduction of sample viscosity
● Elimination of residual DNA during primary stem cell isolation,
biopharma, and bioprocessing procedures
Product FAQs
What is the recommended storage condition for the dsDNase enzyme?
Store the enzyme at -20°C without a defrost cycle to maintain stability and activity.
Can the dsDNase be used in reactions containing RNA or single-stranded DNA?
Yes, dsDNase specifically targets double-stranded DNA while preserving the integrity of single-stranded DNA and RNA molecules.
How is dsDNase activity measured?
One unit of dsDNase activity is defined as the amount of enzyme causing an increase in absorbance at 260 nm of 1.0 in 30 minutes at 37°C and pH 8.0 using herring sperm DNA as a substrate.
What is the recommended concentration of dsDNase in a reaction mixture?
The optimal concentration depends on various factors such as nucleic acid contamination level, temperature, and incubation time. Experimentation is recommended to determine the appropriate conditions for specific applications.
| Quantity | 5000U |
|---|
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