The Avam® DNA Polymerase I Kit is a high-quality enzymatic kit designed for various molecular biology applications, including DNA synthesis, nick-translation, and cDNA synthesis. It offers exceptional performance, purity, and versatility for precise DNA manipulation.
Avam® DNA Polymerase I
$474.00
Purchase this item and get Product Description
Avam® DNA Polymerase I is an indispensable tool for researchers conducting molecular biology experiments requiring DNA synthesis and manipulation. This kit offers researchers high-quality DNA polymerase with exceptional purity and specific activity, ensuring reliable and reproducible results in a wide range of applications. Utilizing a potent mesophilic DNA polymerase, derived from a recombinant E. coli strain carrying the PolA gene, the Avam® DNA Polymerase I Kit facilitates efficient DNA synthesis in the 5′→3′ direction. Additionally, its inherent 3′→5′ and 5′→3′ exonuclease activities enable precise DNA manipulation, allowing for nick-translation during DNA replication processes. The kit includes a 10X VividBlue™ Buffer, meticulously formulated to provide optimal conditions for enzyme activity. This buffer contains essential components such as NaCl, Tris-HCl, MgCl2, and DTT, maintaining the stability and functionality of the DNA polymerase throughout the experimental procedure. Avam® DNA Polymerase I Kit delivers reliable and consistent performance in various molecular biology applications. Its functionality extends to single-stranded exonuclease activity, double-stranded exonuclease activity, and double-stranded endonuclease activity, ensuring precise DNA manipulation and synthesis. Researchers can confidently use the Avam® DNA Polymerase I Kit for DNA labeling by nick translation, second-strand cDNA synthesis, and other DNA manipulation techniques. The kit’s versatility and robust performance make it an essential tool for laboratories conducting molecular biology research.
Kit Contents
● 5000 U of Avam® DNA Polymerase I (10,000 U/mL)
● 10X VividBlue™ Buffer containing: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl2, 10 mM DTT, pH 7.9 at 25°C
Technical Parameters
● Storage temperature: –25°C to –15°C
● Purity: >99%
● Specific activity: 6850 U/mg
● Single-stranded exonuclease: Functional
● Double-stranded exonuclease: Functional
● Double-stranded endonuclease: 200 U; no conversion
● E. coli DNA contamination: 200 U; <10 copies
Applications
Optimal for the following applications:
● DNA labeling by nick translation
● Second-strand cDNA synthesis
● Other DNA manipulation and synthesis techniques
Product FAQs
Can this kit be used for diagnostic purposes?
No, the Avam® DNA Polymerase I Kit is intended for molecular biology applications and is not validated for diagnostic use.
What is the storage temperature for the kit components?
The Avam® DNA Polymerase I should be stored at -25°C to -15°C for optimal stability and performance.
Can this kit be used for nick translation reactions?
Yes, the kit is suitable for DNA labeling by nick translation, enabling precise DNA manipulation and synthesis.
| Quantity | 5000U |
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