AutEx™ 96 Nucleic Acid Extractor

Introduction

The Avam® AutEx™ 96 is a revolutionary tool for molecular biology, offering unmatched precision, speed, and reliability in PCR applications. Its advanced features, including rapid thermal cycling, sophisticated program management, and exceptional temperature control, make it a cornerstone for any lab committed to achieving consistent and reproducible results.

Rapid and Accurate Temperature Control

The AutEx™ 96 is designed for rapid thermal cycling, achieving temperatures up to 100°C in just 1 minute. With a broad temperature control range from room temperature (RT) to 125°C, this system ensures optimal performance across a variety of PCR applications. The temperature accuracy of ±0.25°C (from 35°C to 99.9°C) is critical for precise and consistent results, minimizing the risk of non-specific amplification and ensuring that each reaction proceeds under optimal conditions.

Advanced Cross-Contamination Prevention

Maintaining the integrity of your samples is a top priority with the AutEx™ 96. The built-in UV disinfection lamp provides an effective barrier against cross-contamination by eliminating potential contaminants between runs. This feature, combined with an automatic power-off function after disinfection, ensures that your workspace remains clean and safe. Additionally, the built-in filtration system provides contamination-free ventilation, further reducing the risk of cross-contamination during PCR processes.

Convenient Program and System Management

The AutEx™ 96 is equipped with a multi-functional operating system designed to streamline the process of editing and managing PCR protocols. Users can easily create, edit, and delete program parameters, allowing for custom protocol development tailored to specific research needs. The system supports seamless upgrades via USB, ensuring that it remains compatible with the latest software advancements. Moreover, the ability to import and export programs via USB enhances collaboration and protocol sharing among research teams.

Auto-Check Safety Feature

To minimize operational errors and ensure the accuracy of each PCR run, the AutEx™ 96 includes an auto-check function that verifies the correct positioning of rods, rod sleeves, and well plates before each run. This feature is particularly valuable in high-throughput environments, where the potential for human error is greater. By reducing the likelihood of setup errors, this system helps to maintain the integrity and reliability of your experimental data.

Advanced Mixing Technology

The AutEx™ 96 features advanced mixing technology with speeds up to 10Hz, enabling effective sample lysis and reducing overall experiment runtime. This capability is especially beneficial in applications requiring thorough mixing, such as those involving viscous samples or challenging reagents. The system’s 8 adjustable mixing speeds provide the flexibility needed to meet different experimental requirements, ensuring optimal performance across a wide range of PCR applications.

Compact Structure and Space Efficiency

With its compact and lightweight design, the AutEx™ 96 is engineered to save valuable benchtop space while offering high throughput capabilities. Unlike many other systems that are limited to processing 96 samples per run, this system accommodates adjustable sample throughput from 16 to 96, providing greater flexibility for laboratories with varying workload demands. Its small footprint (364x243x244mm) and lightweight build (8.7kg) make it an ideal choice for labs with limited space, without sacrificing performance or capacity.

High-Throughput Capabilities

The AutEx™ 96 is designed for high throughput, processing up to 96 samples simultaneously with a volume range of 50 – 1000 μl. The system’s processing time ranges from 20 to 40 minutes per run, depending on the reagents used, making it a highly efficient solution for laboratories requiring rapid turnaround times. The system’s 6-plate capacity and compatibility with 96-well deep-well plates further enhance its throughput capabilities, making it suitable for a wide range of PCR applications.

Temperature Management and Performance

Equipped with a heating plate that operates within a temperature range of 37 to 125°C, the AutEx™ 96 offers compatibility with various extraction kits and samples. Its exceptional magnetic intensity, greater than 5000Gs, ensures excellent magnetic attraction performance and short attraction time, making it ideal for magnetic bead-based applications. The system’s temperature accuracy of ±1.5°C guarantees precise control, essential for maintaining the integrity of temperature-sensitive reactions.

Low Noise Operation

Operating at noise levels below 55dB, the AutEx™ 96 provides a quieter laboratory environment, which is especially important in busy settings where multiple instruments are running simultaneously. The system’s low noise output ensures that it does not contribute to the overall noise level in the lab, making it a comfortable and conducive environment for researchers.

Advanced Display and User Interface

The AutEx™ 96 is equipped with a 10-inch high-resolution color touch screen, offering an intuitive and user-friendly interface. The touch screen’s glove-compatible design makes it easy to use, even in a busy lab setting. The system’s intuitive UI interfaces simplify operation, allowing users to quickly navigate between functions and settings. With over 500 program storage capacity, this system provides ample space for storing frequently used protocols, ensuring quick and easy access to your most important programs.

Robust Magnetic Bead Recovery

With a magnetic bead recovery rate as high as 98%, the AutEx™ 96 delivers exceptional performance in applications requiring magnetic separation. The system’s inter-well purification CV of less than 5% ensures consistent and reliable results across all wells, making it an ideal choice for applications where precision is critical.

Comprehensive Contamination Control

The AutEx™ 96’s comprehensive contamination control features include UV light sterilization and built-in HEPA filters, ensuring a sterile and contaminant-free environment for your PCR experiments. These features are particularly valuable in high-sensitivity applications, where even the smallest contamination can compromise results.

Technical Specifications

● Throughput: 1-96 Samples
● Processing Volume: 50 – 1000 μl
● Processing Time: 20 – 40 minutes per run (depending on reagents used)
● Plate Capacity: 6 Plates
● Plate Type: 96-well deep-well plate
● Other Consumables: Magnetic rod sleeves
● Reagent Type: Magnetic bead-based reagent kits (open system)
● Heating Temperature Range: RT to 125°C
● Temperature Accuracy: ±1.5°C
● Mixing Intensities: Adjustable across 8 levels
● Max. Stirring Speed: 10Hz
● Magnetic Bead Recovery: 98%
● Inter-well Purification: CV<5%
● Contamination Control: UV light sterilization, Built-in HEPA filters
● Program Management: Create, Edit, and Delete options available
● Data Storage Capacity: >500 programs
● Display: 10-inch color touch screen
● Extension Ports: 2 USB ports
● Power Supply: 100-240V AC, 50/60Hz
● Noise: <55 dB
● Weight: 35 kg
● Dimensions (WDH): 349mm460mm390mm

Conclusion

The Avam® AutEx™ 96 stands out as a versatile and high-performing tool for any modern molecular biology laboratory. Its advanced features, including rapid thermal cycling, superior temperature control, and robust contamination prevention, ensure reliable and reproducible results across a wide range of PCR applications. Whether you are conducting routine analyses or tackling complex research questions, the AutEx™ 96 provides the precision, efficiency, and convenience needed to achieve your scientific goals.