Amzol™ Lysis Reagent

Amzol™ Lysis Reagent stands as a cornerstone in molecular biology laboratories, offering a reliable solution for efficient RNA extraction from various tissue types. Its advanced formulation, integrating phenol and guanidine-based chemistry, ensures robust lysis, particularly optimized for fatty tissues like brain and adipose tissue. This reagent excels in delivering high-quality RNA, free from contaminants and enzymatic inhibitors, making it indispensable for a wide array of downstream applications.

Developed to address the unique challenges posed by fatty tissues, Amzol™ Lysis Reagent offers an easy-to-follow protocol that simplifies the process of tissue lysis and homogenization. Its compatibility with standard homogenization methods and isopropanol precipitation further enhances its usability, facilitating seamless integration into existing laboratory workflows. By efficiently removing genomic DNA and other impurities, Amzol™ paves the way for enhanced RNA recovery and superior performance in downstream applications.

The organic extraction step employed by Amzol™ Lysis Reagent not only ensures the removal of proteins and DNA but also contributes to the enhancement of RNA recovery. This extraction method, combined with chaotropic disruption, results in the partitioning of RNA into the aqueous phase, thereby facilitating its subsequent isolation and purification. The resultant RNA exhibits exceptional quality, making it suitable for a diverse range of applications, including gene expression analysis, real-time PCR, microarray analysis, and RNA sequencing.

Furthermore, Amzol™ Lysis Reagent’s compatibility with a variety of tissue types underscores its versatility and applicability in diverse research settings. Whether working with fatty tissues like brain and adipose tissue or other tissue types, researchers can rely on Amzol to consistently deliver high yields of RNA with minimal variation.

This reagent’s performance is further validated by supporting data, demonstrating the high quality and yield of RNA obtained from various tissue samples. For instance, total RNA isolated from rat adipose tissue using Amzol™ exhibits superior quality when analyzed with the Agilent 2100 Bioanalyzer, showcasing the effectiveness of this reagent in challenging sample types.

Amzol™ Lysis Reagent emerges as a robust solution for researchers seeking reliable RNA extraction from fatty tissues. With its optimized lysis conditions, compatibility with downstream purification technologies, and proven performance across various tissue types, Amzol facilitates efficient and high-yield RNA extraction, empowering researchers to advance their molecular biology studies with confidence.