Differentiate enteric gram-negative bacilli based on sulfide production, indole formation, and motility using InMicro® Prepared: SIM Medium (Sulfide, Indole, Motility).
InMicro® Prepared: SIM Medium
Price range: $289.00 through $1,370.00
Purchase this item and get Overview
Differentiate enteric gram-negative bacilli based on sulfide production, indole formation, and motility using InMicro® Prepared: SIM Medium (Sulfide, Indole, Motility). Sulkin and Willet reported that H2S detection is an important parameter to differentiate Salmonella and Shigella from other members of the family Enterobacteriaceae. Blazevic used a motility-indole medium to differentiate Klebsiella from Enterobacter and Serratia. Green et al., using smaller quantities of SIM Medium, demonstrated motility results could be interpreted after incubation periods shorter than 18-24 hours.
Sulfide, indole and motility have been combined in one tube for easy use. Indole is detected by adding Kovac’s Reagent or Ehrlich’s Reagent and it combines with para-dimethylaminobenzaldehyde to produce a red complex. The addition of agar forms a semisolid medium which is ideal for the detection of motility.
- Ready to use – Convenience of prepared medium
- Easy to interpret
- Hydrogen Sulfide (S)
- Positive test – Blackening along the line of inoculation
- Negative test – No blackening of the medium
- After addition of Indole (I) reagent
- Positive test – Red color development in the upper portion of the medium
- Negative test – No red color development
- Motility (M)
- Positive test – Diffuse growth outward from the stab line or turbidity throughout the medium
- Negative test – Growth only along the line of inoculation
- Hydrogen Sulfide (S)
This medium contains casein and meat peptones that supply nitrogenous compounds and amino acids necessary for the growth of enteric gram-negative bacilli. Sodium thiosulfate is a sulfur source. Ferric ammonium citrate is an indicator that reacts with H2S produced by sulfate-reducing bacteria to form a black precipitate, ferrous sulfide. Organisms that possess the enzyme tryptophanase degrades tryptophan to produce indole which is detected by the addition of Kovac’s or Ehrlichs’s reagent. The addition of agar results in a semisolid medium suitable for the detection of motility.
Compliance
Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.
Manufacturing Origin
United States
Product Type
Microbiology Media
Format
Tube/Slant
Fill Volume
5 mL
| Product Quantity | Pack of 20, Pack of 100 |
|---|
Related products
-
InMicro® Prepared: Potato Dextrose Agar, pH 5.6
Price range: $112.00 through $1,082.00InMicro® Prepared: Potato Dextrose Agar, pH 5.6 is used to identify, cultivate, and enumerate yeast and molds.Select options This product has multiple variants. The options may be chosen on the product page Show more -
InMicro® Prepared: Levine EMB Agar
Price range: $106.00 through $1,060.00InMicro® Prepared: Levine EMB Agar is a differential, ready-to-use medium designed for the selective isolation of Gram-negative enteric bacteria from clinical specimens.Select options This product has multiple variants. The options may be chosen on the product page Show more -
InMicro® Prepared: Columbia CNA Agar with Sheep Blood Medium
$95.00InMicro® Prepared: Columbia CNA Agar with Sheep Blood Medium is a selective media designed to isolate Gram-positive organisms from clinical samples, traditionally used to identify staphylococci, streptococci, and S. pneumoniae.Select options This product has multiple variants. The options may be chosen on the product page Show more -
InMicro® Prepared: Anaerobic PEA Blood Agar with 5% Sheep Blood
Price range: $126.00 through $1,260.00InMicro® Prepared: Anaerobic PEA Blood Agar (Anaerobic Phenylethyl Alcohol Agar) with 5% Sheep Blood is a selective, ready-to-use medium designed to isolate and cultivate anaerobic organisms, including fastidious strains.Select options This product has multiple variants. The options may be chosen on the product page Show more
Updating…