Isolate Salmonella spp. using selective enrichment broth InMicro® Prepared: Selenite Broth with Cystine.
InMicro® Prepared: Selenite Broth with Cystine is a selective enrichment medium for isolation of Salmonella. Leifson formulated Selenite Broth for enrichment of Salmonella from sources such as feces, urine, and food. Selenite was found to inhibit coliforms and fecal streptococci, however after 12-18 hours of incubation the breakthrough of inhibited strains would occur. Addition of L-cystine as an enrichment agent improves the recovery of Salmonella. Bacteria that reduce selenite produce alkali and increase the pH. The acid produced by lactose fermentation causes a decrease in pH and results in a neutral or slightly decreased pH in the medium. Selenite Cystine Broth is used in epidemiological studies to detect organisms from asymptomatic or convalescent patients and for recovery of Salmonella and Shigella when it occurs in low numbers in certain specimens.
This medium contains casein peptone that supplies nitrogenous substances and carbon compounds necessary for bacterial growth. L-cystine is the enrichment agent that improves recovery of the organisms. Sodium phosphate maintains a stable pH and lessens the toxicity of the selenite. Selective agent sodium selenite inhibits coliforms, enterococci, and other gram-positive organisms.
Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.
Microbiology Media
Tube/Slant
Product Quantity | 100 x 9 mL, 100 x 10 mL |
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