InMicro® Prepared: R2A Agar is designed to enumerate and cultivate heterotrophic bacteria from potable water using R2A Agar.
Enumerate and cultivate heterotrophic bacteria from potable water using InMicro® Prepared: R2A Agar. The growth of stressed and chlorine-tolerant bacteria recovered from treated waters is stimulated using a less nutritious medium, lower incubation temperatures, and longer incubation times. Information on water quality, such as heterotrophic plate counts, may be used to evaluate the efficiency of treatment processes.
Use InMicro® Prepared: R2A Agar for enumeration and cultivation of heterotrophic bacteria from potable water.
Reasoner and Geldreich of the U.S. Environmental Protection Agency developed R2A Agar for the isolation and recovery of aerobic and facultative heterotrophic bacteria from treated potable water samples. R2A Agar was found to be superior to Plate Count Agar when using the pour plate, spread plate, or membrane filter techniques. In this medium the low nutrients give higher counts in treated potable waters as compared to a highly nutritious medium because these bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium.
This medium contains peptone that supplies nitrogenous compounds and amino acids necessary for growth. Dextrose is added as a carbon source of energy. Yeast extract supplies B-complex vitamins and serves as a growth enhancer. Addition of soluble starch as a neutralizer and sodium pyruvate stimulates the growth of stressed bacteria. Buffering agents such as dipotassium phosphate and magnesium sulfate maintain osmotic equilibrium.
Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.
Agar
Media Format | Monoplate, Bottle |
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Product Quantity | Pack of 10, Pack of 100, 10 x 500 mL |