Facilitate efficient RNA polyadenylation with the Avam® Poly (A) Polymerase, designed to catalyze the addition of AMP from ATP to the 3’ hydroxyl of RNA molecules, enabling various molecular biology applications.
Avam® Poly (A) Polymerase
$474.00
Purchase this item and get Product Description
The Avam® Poly (A) Polymerase includes Poly(A) Polymerase enzyme and a specialized reaction buffer, meticulously formulated to enhance RNA polyadenylation processes. Avam® Poly (A) Polymerase catalyzes the attachment of ATP-derived AMP to the 3’ hydroxyl terminus of RNA, a crucial step in generating poly(A)-tailed RNA. The enzyme, derived from an E. coli expression system, operates in a template-independent manner and requires Mg2+ ions for activity. The provided reaction buffer ensures optimal conditions for efficient polyadenylation reactions. This kit empowers researchers to perform polyadenylation with precision and reliability, enabling applications such as poly(A) tailing, RNA labeling, and mRNA therapeutics.
Kit Contents
● Avam® Poly (A) Polymerase (1000 U, 5000 U/mL)
● 10X Poly (A) Polymerase Reaction Buffer
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 53.8 Kilo Daltons
● Purity: >95%
● Specific activity: >20,000 U/mg
● Single-stranded exonuclease: <5% released
● Double-stranded exonuclease: <1% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
● RNase contamination: No detectable non-specific RNase
Applications
The Avam® Poly (A) Polymerase Kit is suitable for a wide range of molecular biology applications, including:
● Polyadenylation of RNA molecules
● 3’ end RNA labeling
● mRNA therapeutics
Product FAQs
What is the storage temperature for the Avam® Poly (A) Polymerase Kit?
The kit should be stored at temperatures ranging from –25°C to –15°C to maintain enzyme stability.
How is the specific activity of Avam® Poly (A) Polymerase determined?
Specific activity is measured using a two-fold serial dilution method, assessing the enzyme’s ability to incorporate ATP into acid-insoluble material.
What is the principle behind Avam® Poly (A) Polymerase activity?
Avam® Poly (A) Polymerase catalyzes the addition of AMP from ATP to the 3’ hydroxyl terminus of RNA molecules, a process essential for polyadenylation.
| Quantity | 1000U |
|---|
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