Ecose™ Glasgow's MEM (GMEM)

Ecose™ Glasgow’s MEM (GMEM)

$48.81

Glasgow’s MEM (GMEM) was originally developed by Ian McPherson and Michael Stoker as a modification of Eagle’s Minimal Essential Medium.

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Product Description

Glasgow’s MEM (GMEM) was originally developed by Ian McPherson and Michael Stoker as a modification of Eagle’s Minimal Essential Medium. It was used to study the genetic factors that affected cell competence. Glasgow’s MEM was developed for use with kidney cell lines, such as BHK-21.

This Glasgow’s MEM is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Tryptose Phosphate Broth

The complete formulation is available.

Using Glasgow’s MEM
Glasgow’s MEM is unique from other media as it contains twice the concentration of amino acids and vitamins compared to the original Basal Medium Eagle, and is used without serum. Glasgow’s MEM was originally formulated with 10% tryptose phosphate broth. Glasgow’s MEM contains no proteins, lipids, or growth factors. Therefore, Glasgow’s MEM requires supplementation with 10% tryptose phosphate broth. Glasgow’s MEM uses a sodium bicarbonate buffer system (2.75 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Content And Storage

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Concentration

1 X

Product Type

MEM (Minimum Essential Medium)

Sterility

Sterile-filtered

Cell Line

BHK-21

Classification

Animal Origin-free

Form

Liquid

With Additives

High Glucose, Glutamine, Phenol Red

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Quantity

500 mL

Product Quantity

500 mL
SKU: CBM7215 Category:
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