Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Ecose™ RPMI 1640 Medium (Dutch modification)
$43.51
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Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Ecose™ RPMI 1640 modifications for a range of cell culture applications.
This RPMI is modified as follows:
With: Phenol Red, HEPES, Low Sodium Bicarbonate
Without: L-glutamine
The complete formulation is available.
Ecose™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. This RPMI formulation has the Dutch modification (addition of HEPES, with sodium bicarbonate lowered to 1 g/L).
Product Intended Use
For Research Use Only. Not for use in diagnostic procedures.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (1.0 g/L) and therefore requires a 5-7% CO2 environment to maintain physiological pH.
Content And Storage
Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Type
Leukemic Cells
Concentration
1 X
Product Type
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterility
Sterile-filtered
Cell Line
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Classification
Animal Origin-free
Form
Liquid
Serum Level
Standard Serum Supplementation
With Additives
HEPES, Phenol Red, Low Sodium Bicarbonate
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
500 mL
| Product Quantity | 500 mL |
|---|
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