Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Ecose™ RPMI 1640 Medium, powder, HEPES
Price range: $101.00 through $113.00
Purchase this item and get Product Description
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas.
This RPMI is modified as follows:
With: L-glutamine, Phenol Red, HEPES
Without: Sodium Bicarbonate
Ecose™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Ecose™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.
Content And Storage
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Cell Type
Leukemic Cells
Product Type
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterility
Sterile-filtered
Cell Line
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Classification
Animal Origin-free
Form
Powder
Serum Level
Standard Serum Supplementation
With Additives
Glutamine, HEPES, Phenol Red
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
10 x 1 L
| Product Quantity | 10 x 1 L, 10 L |
|---|
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