Ecose™ Keratinocyte-SFM (1X), with L-glutamine, without calcium chloride

Ecose™ Keratinocyte-SFM (1X) is a specialized, serum-free medium designed to support the growth and propagation of human epidermal keratinocytes, engineered for optimal performance in environments that require precise calcium control. With the addition of L-glutamine and the omission of calcium chloride, Ecose™ Keratinocyte-SFM allows researchers to tightly regulate calcium levels according to experimental needs, making it particularly suitable for studies on
keratinocyte differentiation, cell adhesion, and calcium-sensitive cellular processes. Ideal for applications in dermatology, wound healing, and skin regeneration research, Ecose™ Keratinocyte-SFM provides a consistent, defined culture environment for robust and reproducible results.

Product Features and Benefits:
● Calcium-Free Design for Customizable Control: The absence of calcium chloride offers full control over calcium concentrations, making it possible to tailor calcium levels to suit specific stages of keratinocyte culture, such as differentiation, while enabling a more precise examination of calcium’s role in cell signaling and function.
● Enriched with L-Glutamine: The inclusion of L-glutamine, an essential amino acid that supports keratinocyte growth and metabolic function, ensures high cell viability and vigor, contributing to prolonged culture sustainability and enhanced cellular health.
● Serum-Free Formulation: Eliminates the batch variability and unknown factors associated with serum, supporting a reproducible and defined cell culture environment. This serum-free design enhances the relevance of experimental outcomes, especially in high-sensitivity assays where
consistent conditions are vital.
● Enhanced Stability and Reproducibility: Ecose™ Keratinocyte-SFM is manufactured under strict quality control measures to ensure batch-to-batch consistency, making it ideal for studies requiring repeatable, reliable conditions across multiple experimental cycles.
● Optimized for Keratinocyte Proliferation and Maintenance: Provides a balanced mixture of nutrients, growth factors, trace elements, and vitamins to support keratinocyte health, including high viability and ideal morphology, without promoting fibroblast growth, allowing for the growth
of pure keratinocyte cultures.

Applications: Ecose™ Keratinocyte-SFM (1X) serves as an essential medium in a
wide range of skin biology and dermatological research applications, offering
controlled conditions that suit both basic research and applied clinical studies:
● Keratinocyte Differentiation and Calcium Signaling: With a calcium-free base, this medium is ideal for examining the effects of calcium in keratinocyte differentiation, adhesion, and signaling pathways, making it essential for studies on skin physiology and development.
● Tissue Engineering and Regenerative Medicine: Supports keratinocyte expansion and maintenance, aiding the development of skin grafts, bioengineered skin constructs, and wound healing models. 
● Pharmaceutical and Cosmetic Testing: Enables a serum-free environment that ensures reproducibility in toxicology and efficacy testing of drugs or cosmetic compounds affecting keratinocyte function and skin health.
● Molecular and Genetic Studies: Provides a stable and defined environment for investigating gene expression, protein production, and pathway activation in keratinocytes, ensuring experimental accuracy and minimal variability.

Ecose™ Keratinocyte-SFM (1X), with its calcium-free formulation and essential L-glutamine supplementation, is designed to meet the demanding standards of skin biology and keratinocyte research. Its reliable, serum-free environment supports high-performance applications, ensuring robust, reproducible, and insightful results for advanced research into human keratinocyte biology.