Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells.
Ecose™ Basal Medium Eagle (BME)
$37.74
Purchase this item and get Product Description
Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.
This BME is manufactured as follows:
- With: Phenol Red
- Without: L-glutamine, HEPES
The complete formulation is available.
BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow’s Medium and Dulbecco’s Modified Eagle’s Medium. This BME formulation contains Earle’s salts for use in a CO2 incubator.
BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Content And Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Concentration
1 X
Product Type
Basal Medium Eagle (BME)
Sterility
Sterile-filtered
Cell Line
HeLa, mouse fibroblasts, WI-38 and MRC-5
Classification
Animal Origin-free
Form
Liquid
With Additives
Low Glucose, Phenol Red
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
500 mL
| Product Quantity | 500 mL |
|---|
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