Ecose™ RPMI 1640 Medium

Product Description

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications.

This RPMI is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Content And Storage

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Type

Leukemic Cells

Concentration

1 X

Product Type

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)

Sterility

Sterile-filtered

Cell Line

HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas

Classification

Animal Origin-free

Form

Liquid

Serum Level

Standard Serum Supplementation

With Additives

Glutamine, Phenol Red

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Quantity

500 mL