A specialized, low-protein medium optimized for endothelial cell culture, designed for applications requiring precise control over glutamine supplementation.
Ecose™ MCDB 131 Medium, no glutamine
$73.50
Purchase this item and get Product Description
MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HUVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells.
Using MCDB 131
MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, hydrocortisone, glutamine, and low levels of Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Content And Storage
Storage conditions: 2–8°C (protect from light)
Shelf life: 12 months from date of manufacture
Cell Type
Human Microvascular Endothelial Cells (HMVEC), Human Omental Microvascular Cells, Hepatocytes, Myocytes, Smooth Muscle Cells
Product Type
MCDB 131 Medium
Sterility
Sterile-filtered
Form
Liquid
With Additives
Low Glucose, Phenol Red, Sodium Pyruvate
Quantity
500 mL
| Product Quantity | 500 mL |
|---|
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